Impact of air pollution and genotype variability on DNA damage in Prague policemen
| Autor: | Zdena Lnenickova, Bozena Novotna, Irena Chvatalova, Radim J. Sram, Jan Topinka, Ivo Solansky |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Genotype DNA damage Physiology Biology Toxicology medicine.disease_cause Occupational Exposure Surveys and Questionnaires Cytochrome P-450 CYP1A1 medicine Humans Lymphocytes Polycyclic Aromatic Hydrocarbons Methylenetetrahydrofolate Reductase (NADPH2) Carcinogen Czech Republic Epoxide Hydrolases Inhalation exposure Polymorphism Genetic General Medicine Carcinogens Environmental Police Comet assay Oxidative Stress Case-Control Studies Particulate Matter Comet Assay Seasons Oxidative stress DNA Damage Environmental Monitoring Nucleotide excision repair Blood sampling |
| Zdroj: | Toxicology Letters. 172:37-47 |
| ISSN: | 0378-4274 |
| DOI: | 10.1016/j.toxlet.2007.05.013 |
| Popis: | DNA integrity was analyzed in the lymphocytes of 65 non-smoking city policemen during January and September 2004 using the comet assay combined with excision repair enzymes. Information about inhalation exposure was obtained by (1) stationary monitoring of PM2.5 and carcinogenic polycyclic aromatic hydrocarbons (cPAHs) during the sampling periods and (2) personal exposure monitoring of cPAHs 48 h before blood sampling. The data were completed by a lifestyle questionnaire. Regardless of the season of the year, policemen working outdoors (exposed group) exhibited higher levels of DNA damage than those working indoors (controls). Within the exposed group, the levels of both unspecified and oxidative DNA damage detected in January significantly exceeded those found in September. The controls did not show analogous inter-seasonal variability. The winter levels of oxidative DNA damage positively correlated with exposure to cPAHs, probably reflecting increased oxidative stress as a result of high concentrations of PM2.5. In comparison with the wild type genotype, the carriers of at least one mutated allele, CYP1A1*2C (Ile/Val), MTHFR 2656 or MS 2656, and the EPHX1-medium phenotype appeared to be more susceptible specifically to the induction of oxidative DNA damage, while the p53 MspI mutation predisposed the carrier to a higher incidence of both breaks and oxidative lesions in DNA. In contrast, GSTM1-null and vitamin C tended rather to protect DNA integrity. |
| Databáze: | OpenAIRE |
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