Antifungal activity of different molecular weight chitosans against planktonic cells and biofilm of Sporothrix brasiliensis
| Autor: | Lana Glerieide Silva Garcia, Xhaulla Maria Quariguasi Cunha Fonseca, Rossana de Aguiar Cordeiro, Débora de Souza Collares Maia Castelo-Branco, Raimunda Sâmia Nogueira Brilhante, Rodrigo Silveira Vieira, Glaucia Morgana de Melo Guedes, Waldemiro de Aquino Pereira-Neto, José Júlio Costa Sidrim, Marcos Fábio Gadelha Rocha |
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| Rok vydání: | 2019 |
| Předmět: |
Antifungal
medicine.drug_class macromolecular substances 02 engineering and technology Biochemistry Chitosan 03 medical and health sciences chemistry.chemical_compound Structural Biology medicine Sporothrix schenckii Humans Food science Crystal violet Molecular Biology 030304 developmental biology 0303 health sciences biology Chemistry Sporothrix Broth microdilution technology industry and agriculture Biofilm food and beverages General Medicine equipment and supplies 021001 nanoscience & nanotechnology biology.organism_classification Plankton Sporotrichosis carbohydrates (lipids) Molecular Weight Acetylation Biofilms Hydroxide 0210 nano-technology |
| Zdroj: | International journal of biological macromolecules. 143 |
| ISSN: | 1879-0003 |
| Popis: | Sporotrichosis, caused by Sporothrix schenckii complex species, is the most prevalent subcutaneous mycosis in many areas of Latin America. Chitosan has been used as an antifungal agent; however the effects of the molecular weight (MW) of chitosan (i.e. high (HMW), medium (MMW) and low (LMW) molecular weight chitosan) on S. brasiliensis has not been well described, particularly on biofilms. Effects on the planktonic form activity of S. brasiliensis were quantified by broth microdilution, while anti-biofilm activity was quantified by measuring metabolic activity via XTT (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide and biomass formation (crystal violet). The molecular weight of chitosan modulated its effect on the planktonic form of S. brasiliensis, presenting lower MIC values for LMW chitosan. With regards both the adhesive and mature phases of biofilm, the LMW chitosan reduced biomass and metabolic activity most effectively. This study confirms the effects of the molecular weight and deacetylation degree of chitosan on its antifungal properties for potentially pathogenic fungi. |
| Databáze: | OpenAIRE |
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