A multifunctional bicupin serves as precursor for a chromosomal protein of Pisum sativum seeds
| Autor: | Josefa Castillo, M. Isabel Rodrigo, Ainhoa Genovés, Luis Franco |
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| Rok vydání: | 2005 |
| Předmět: |
Models
Molecular Physiology Sequence analysis Chromosomal Proteins Non-Histone Molecular Sequence Data Plant Science Response Elements DNA-binding protein Pisum Sativum Gene Expression Regulation Plant Sequence Analysis Protein Gene expression Storage protein Amino Acid Sequence RNA Messenger Protein Precursors Promoter Regions Genetic Gene Plant Proteins chemistry.chemical_classification Messenger RNA biology Peas food and beverages biology.organism_classification Biochemistry chemistry Multigene Family Protein Biosynthesis Seeds Protein Processing Post-Translational Sequence Alignment Abscisic Acid |
| Zdroj: | Journal of experimental botany. 56(422) |
| ISSN: | 0022-0957 |
| Popis: | The fact that the psp54 gene codes for p16, a seed chromatin protein of Pisum sativum, has been described previously. In the present paper it is shown that p54, the p16 precursor, also exists as a free polypeptide in pea and that it also yields p38, a second polypeptide from the N-terminal region of p54, which is co-localized at a subcellular level with p16. By using antibodies against pea p16 and p38, it was found that these proteins are present in the members of the tribe Viciae examined. Sequence analysis and 3D modelling indicates that p54 proteins belong to the cupin superfamily, and that they are related to sucrose binding proteins and, to a lesser extent, to vicilin-type seed storage proteins. Nevertheless, several distinctive characteristics of psp54 expression have been found: (i) the gene is differentially induced by ABA and several stress situations, in accordance with the presence of putative separate ABA and stress responsive elements in its promoter; (ii) the proteins are present in pods and seed coats, tissues of maternal origin; and (iii) p54 mRNA accumulates in the dry seeds. In view of both the functional properties of p54-derived proteins and the features of the psp54 gene expression, it is concluded that p54 represents a novel class within the cupin superfamily. |
| Databáze: | OpenAIRE |
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