Relation between expression pattern of p53 and survivin in cutaneous basal cell carcinomas
| Autor: | Desanka Vybohova, Erika Halasova, Silvia Rybárová, Marian Adamkov, Marian Bencat, Július Rajčáni, S Galbavy |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
p53
Adult Male Skin Neoplasms Cell division Tumor suppressor gene Survivin Cell Count Biology carcinoma Inhibitor of apoptosis Inhibitor of Apoptosis Proteins basal Carcinoma medicine Humans Basal cell carcinoma Aged Aged 80 and over General Medicine cell Middle Aged medicine.disease Subcellular localization Protein Transport Basic Research Apoptosis Carcinoma Basal Cell immunohistochemistry Cancer research Female Tumor Suppressor Protein p53 Subcellular Fractions |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research Scopus-Elsevier |
| ISSN: | 1643-3750 1234-1010 |
| Popis: | Summary Background The tumor suppressor gene p53 is a key regulator of cell division and/or apoptosis. Survivin is a multifunctional member of the inhibitor of apoptosis family. Survivin and p53 represent diametrically opposed signals that influence the apoptotic pathway. Material/Methods To determine the role of p53 and survivin in basal cell carcinoma (BCC), we evaluated the expression pattern of both proteins with regard to the percentage of positively immunostained tumor cells, the intensity of staining, and subcellular localization among 31 subjects with BCC. Results Overexpression of p53 protein was found in 28 of 31 cases (90.3%), whereas survivin accumulation was seen in 27 (87.1%). For p53, moderate and/or strong immunoreactivity was seen in 20 of 28 cases (71.4%), and 26 of 28 cases (92.9%) showed more than 25% reactive tumor cells. Nuclear p53 staining was detected in 23 of 28 cases (82.1%), whereas combined nuclear and cytoplasmic localization was found in only 5 of 28 cases (17.9%). Survivin revealed mild intensity of immuno-reaction in 22 of 27 cases (71%), and 25 of 27 cases (92.6%) showed less than 25% labeled tumor cells. Combined nuclear and cytoplasmic survivin localization was present in 26 of 27 cases (96.3%). Statistically significant differences were detected in the assessed expression parameters between those proteins. Conclusions Our results suggest that overexpression of wild type p53 protein may suppress the expression of survivin and its antiapoptotic activity in BCC cells. |
| Databáze: | OpenAIRE |
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