Pristane induced gene activation
| Autor: | Clinton Jones, Marvin A. Cuchens, Lenora R. Garrett |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Transcriptional Activation Gene Expression Simian virus 40 Biology Transfection Toxicology 12-O-Tetradecanoylphorbol-13-acetate medicine.disease_cause Gene Expression Regulation Enzymologic Cell Line Mice chemistry.chemical_compound Gene expression medicine Animals Cell Line Transformed Regulation of gene expression integumentary system Terpenes Pristane 3T3 Cells General Medicine Molecular biology chemistry Biochemistry Genes Bacterial Cell culture Tetradecanoylphorbol Acetate Carcinogens Carcinogenesis Plasmids |
| Zdroj: | Chemico-Biological Interactions. 81:119-130 |
| ISSN: | 0009-2797 |
| DOI: | 10.1016/0009-2797(92)90030-o |
| Popis: | Studies were performed to examine the effects of 2,6,10,14-tetramethyl pentadecane (pristane) versus 12-O-tetradecanoylphorbol 13-acetate (TPA) on the activation of the CAT gene under the regulatory control of viral promoter/enhancer elements transfected into NIH-3T3, CV-1 and COS-7 cells. The results of these studies demonstrated that (1) pristane or TPA induced trans-activation of SV2cat, HIVcat, RSVcat and MMTVcat in cells transfected with each respective plasmid construct, (2) only pristane induced activation of pA10cat and pOSP/11 and (3) neither TPA nor pristane trans-activated pSV0cat. Furthermore, treatment with either pristane or TPA elicited changes in the morphology of each of the cell lines. Collectively these results indicate that pristane is a potent inducer of gene expression and exhibits similar characteristics as the tumor promoter, TPA. |
| Databáze: | OpenAIRE |
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