Metallothionein Immunolocalization in Actinic Skin Nonmelanoma Carcinomas
| Autor: | Paulo Cesar Borges Júnior, Ademir Rocha, Foued Salmen Espindola, Adriano Mota Loyola, Sérgio Vitorino Cardoso, Alceu L. C. Berbet, Rosy Iara Maciel de Azambuja Ribeiro |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Skin Neoplasms Histology Ultraviolet Rays medicine.drug_class Monoclonal antibody Pathology and Forensic Medicine Basal (phylogenetics) medicine Humans Metallothionein Basal cell carcinoma Aged integumentary system Chemistry medicine.disease Immunohistochemistry Epithelium Medical Laboratory Technology medicine.anatomical_structure Carcinoma Basal Cell Carcinoma Squamous Cell Cancer research Female Skin cancer Immunostaining |
| Zdroj: | Applied Immunohistochemistry & Molecular Morphology. 15:165-169 |
| ISSN: | 1541-2016 |
| DOI: | 10.1097/01.pai.0000213115.27978.73 |
| Popis: | Basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) are the most frequent skin cancer. Its pathogeny is linked to genotoxic effects of actinic radiation exposure, especially to ultraviolet wavelength. Metallothionein (MT) is a low-molecular weight protein with high affinity for heavy metal. Its intracellular function has been related to heavy metals and free-radical detoxification, although many studies linked MT to protective action against actinic mutagenesis. In other way, overexpression in malignant tumors has been related to worse prognosis. We aimed to evaluate MT immunohistochemical expression in skin cancer associated to actinic radiation. Twenty-six BCC cases, 20 SCC, and 6 normal skin fragments were investigated. Immunohistochemical assay were performed by streptavidin-biotin-peroxidase technique with standard monoclonal antibody (E9). In normal skin, immunostaining was observed in basal layer of the epithelium. In the epithelium adjacent to tumors, suprabasal layer was also intensely labeled. Mean MT immunostaining indices were 18.5+21.2% for BCC and 69.1+14.4% for SCC. This difference was statistically significant. Higher MT expression in SCC as compared with BCC suggests association with tumoral aggressiveness. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|