Effects of Spermine and Spermidine supplemented extenders on post-thaw Spermatological Parameters in Stallion Semen Cryopreservation
Autor: | Kemal Tuna Olgac, Ergun Akçay |
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Rok vydání: | 2021 |
Předmět: |
Male
Spermidine Spermine Semen General Biochemistry Genetics and Molecular Biology Cryopreservation law.invention Andrology 03 medical and health sciences chemistry.chemical_compound Cryoprotective Agents 0302 clinical medicine Capacitation law Animals Humans Horses 030219 obstetrics & reproductive medicine Extender 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine Spermatozoa 040201 dairy & animal science Semen cryopreservation chemistry Sperm Motility Female General Agricultural and Biological Sciences Polyamine Semen Preservation |
Zdroj: | Cryobiology. 100:72-76 |
ISSN: | 0011-2240 |
Popis: | In this study, the effects of polyamines, Spermine and Spermidine, on long-term preservation and post-thaw spermatological parameters were evaluated. Moreover, determination of the most suitable polyamine and its dose that can be added to standard extenders were aimed. Four adult Arabian stallions were used in the study. Five ejaculates were collected from each of four stallions via artificial vagina two days interval. Each ejaculate was divided into 13 aliquots. INRA96 (95,5%), egg yolk (2%), and glycerol (2,5%) were used as a control extender. Extenders of experimental groups were prepared with different doses of Spermine and Spermidine (0,1 mg/ml; 0,2 mg/ml; 0,4 mg/ml; 1 mg/ml; 2 mg/ml; 4 mg/ml). Stallion semen that were cryopreserved with Control and experimental extenders were evaluated in terms of Total Motility, Progressive Motility, Plasma Membrane Integrity, Capacitation Index, Acrosome Integrity and DNA Fragmentation Index. At the end of the evaluations, it was determined that 0,2 mg/ml Spermine and 0,4 mg/ml Spermidine showed better Total Motility and Progressive Motility, numerically. On the other hand, it was observed that 4 mg/ml Spermine and Spermidine had the lowest statistically significant values (p 0,001). While statistically similar differences were obtained between groups in terms of the Plasma Membrane and Acrosome Integrity, it was determined that all experimental groups had lower and statistically significant values in terms of Capacitation and DNA Fragmentation Index (p 0,001). As result, it was observed that the stallion semen cryopreservation success can be increased by the addition of 1-2 mg/ml Spermine that had effective protection on Capacitation and DNA Fragmentation Index without damaging other spermatological properties. |
Databáze: | OpenAIRE |
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