CD10/NEP is expressed on Thy-1low B220+ murine B-cell progenitors and functions to regulate stromal cell-dependent lymphopoiesis
| Autor: | G, Salles, C Y, Chen, E L, Reinherz, M A, Shipp |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
B-Lymphocytes
Membrane Glycoproteins Base Sequence Molecular Sequence Data fungi Immunology Gene Expression Bone Marrow Cells Cell Differentiation Cell Biology Hematology Hematopoietic Stem Cells Polymerase Chain Reaction Biochemistry Cell Line Hematopoiesis Mice immune system diseases hemic and lymphatic diseases Antigens Surface Animals RNA Thy-1 Antigens Neprilysin Cell Line Transformed |
| Zdroj: | Blood. 80:2021-2029 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v80.8.2021.bloodjournal8082021 |
| Popis: | To further characterize the function of the common acute lymphoblastic leukemia antigen (CALLA; CD10, neutral endopeptidase 24.11, NEP) in early lymphoid development, we have identified murine lymphoid progenitors expressing CD10/NEP and analyzed the effects of inhibiting the enzyme in in vitro assays of murine lymphoid differentiation. CD10/NEP transcripts and enzymatic activity were primarily restricted to the subpopulation of murine lymphoid progenitors, termed pro-B cells, which were isolated from bone marrow (BM) and modified Whitlock- Witte cultures and defined by coexpression of B220 and low levels of Thy-1. CD10/NEP transcripts and cell surface enzymatic activity were also detected in BM stromal cells known to support the development of B- lymphoid progenitors. In contrast, Abelson and H-ras transformed pre-B- cell lines were CD10/NEP- as were Thy-1-B220+ pre-B cells from BM and modified Whitlock-Witte cultures and Thy-1lowLin- (B220-Mac-1-GR-1-Ly- 2/3-) uncommitted hematopoietic progenitors from BM. The expression of CD10/NEP on murine pro-B cells and BM stromal cells suggests a role for the enzyme in early B-cell ontogeny. In modified Whitlock-Witte cultures in which Thy-1lowLin- progenitors plated on BM stromal cells differentiate into Thy-1lowB220+ pro-B and Thy-1-B220+ pre-B cells, the addition of specific CD10/NEP inhibitors increased the number of lymphoid colonies at days 5 through 7 by 34% (P < .001). The results suggest that CD10/NEP participates in the regulation of the earliest stages of stromal cell-dependent B lymphopoiesis. |
| Databáze: | OpenAIRE |
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