GT1-7 Cell-Based cytoxicity screening assay on 96-well microplates as a platform for the safety assessment of genetically modifiedGerbera hybridaextracts
| Autor: | Pia Vuorela, Adyary Fallarero, Teemu H. Teeri, Miia Ainasoja, Malena Sandberg |
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| Rok vydání: | 2009 |
| Předmět: |
Crops
Agricultural Gerbera Health Toxicology and Mutagenesis Drug Evaluation Preclinical Genetically modified crops Asteraceae Toxicology Risk Assessment 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Gene Expression Regulation Plant Propidium iodide Cytotoxicity Cells Cultured 030304 developmental biology Dosage Forms Pharmacology 0303 health sciences Chemical Health and Safety biology business.industry Medical screening Public Health Environmental and Occupational Health Screening assay General Medicine Plants Genetically Modified biology.organism_classification Genetically modified organism Biotechnology chemistry Biochemistry Consumer Product Safety Genetic Engineering business 030217 neurology & neurosurgery Cell based |
| Zdroj: | Drug and Chemical Toxicology. 32:120-127 |
| ISSN: | 1525-6014 0148-0545 |
| DOI: | 10.1080/01480540802593857 |
| Popis: | In this investigation, a GT1-7 cell-based cytotoxicity screening assay in 96-well microplates was set up. The assay, using propidium iodide fluorescence, was proven to be reliable, with good quality (Z' = 0.51) and low plate-to-plate and day-to-day variations. Further on, a library containing extracts from 227 genetic modification (GM) Gerbera hybrida and 42 Gerbera varieties was screened; however, no differences between them were found. Based on these findings, we propose the use of the current assay within the first-tier screening studies of large collections. Also, these results provide valuable information for GM Gerbera risk-assessment purposes and offer a model for the toxicity cell-based screening of GM crops. |
| Databáze: | OpenAIRE |
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