Cell therapy in experimental model of inflammatory bowel disease
| Autor: | Renata Aparecida de Camargo Bittencourt, Luiz Claudio Di Stasi, Natalia Langenfeld Fuoco, Thaís Helena da Motta Conceição, Monica Yonashiro Marcelino, Ana E.V. Quaglio, Bruna Cambraia Garms, João Tadeu Ribeiro-Paes |
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| Přispěvatelé: | Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp), Universidade Paulista Institute of Health Sciences, Universidade Federal da Grande Dourados School of Biological and Environmental Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Population Adipose tissue Inflammation RC799-869 Stem cells Inflammatory bowel disease Cell therapy 03 medical and health sciences 0302 clinical medicine Intestinal mucosa Medicine education education.field_of_study business.industry Gastroenterology Doença Inflamatória Intestinal Diseases of the digestive system. Gastroenterology medicine.disease Terapia Celular Tecido Adiposo Transplantation 030220 oncology & carcinogenesis 030211 gastroenterology & hepatology Stem cell medicine.symptom business Células-tronco |
| Zdroj: | Journal of Coloproctology (Rio de Janeiro), Volume: 35, Issue: 1, Pages: 20-27, Published: MAR 2015 Journal of Coloproctology, Vol 35, Iss 1, Pp 20-27 (2015) Journal of Coloproctology (Rio de Janeiro) v.35 n.1 2015 Journal of Coloproctology (Rio de Janeiro. Online) Sociedade Brasileira de Coloproctologia (SBCP) instacron:SBCP SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2015-08-21T17:54:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-03-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) A Doença Inflamatória Intestinal (DII), consistindo principalmente da doença de Crohn e retocolite ulcerativa, é uma condição inflamatória da mucosa que pode acometer qualquer segmento do trato gastrointestinal. Apesar das terapias existentes resultarem na melhora dos sintomas e da qualidade de vida dos pacientes, não há nenhum tratamento curativo. O tratamento cirúrgico envolve procedimentos complexos associados a altas taxas de morbimortalidade. Neste contexto, a terapia celular com células-tronco desponta como opção de tratamento potencialmente promissora. Em função destes aspectos, pretendeu-se, no presente estudo, verificar a eficácia do transplante de células-tronco derivadas do tecido adiposo (ASC) em ratos com inflamação intestinal induzida por ácido trinitrobenzenosulfonico (TNBS). As ASCs foram obtidas por dissociação mecânica do tecido adiposo da região inguinal de ratos e processadas para cultivo. Os animais foram avaliados, considerando-se os aspectos clínicos e bioquímicos, além de análises macroscópica, microscópica e histológica. No modelo de inflamação intestinal induzida por TNBS, a infusão de ASCs reduziu significativamente a presença de aderências entre o cólon e órgãos adjacentes, bem como diminuiu a atividade da mieloperoxidase (MPO), um marcador da infiltração de neutrófilos na mucosa lesada. Os resultados obtidos permitem concluir que a terapia celular com ASCs pode promover e/ou acelerar o processo de regeneração da mucosa intestinal inflamada. Inflammatory bowel disease, which mainly involves Crohn's disease and ulcerative rectocolitis, is an inflammatory condition of the mucosa that can afflict any segment of the gastrointestinal tract. Despite the fact that the existing therapies result in improvement in patient's symptomatology and quality of life, there is no curative treatment. Surgical treatment involves complex procedures associated with high morbidity and mortality rates. In this context, cell therapy with stem cells has emerged as a treatment with broad potential applicability. In this study, we intended to verify the efficacy of transplantation of adipose tissue-derived stem cells in rats with intestinal inflammation induced by trinitrobenzenesulfonic acid. The cell population was isolated from the adipose tissue of inguinal region of rats and processed for culture by mechanical dissociation. The animals were evaluated with respect to clinical and biochemical aspects, as well as by macroscopic, microscopic and histological analyses. In the experimental model of bowel inflammation by 2,4,6-trinitrobenzenesulfonic acid, the infusion of adipose tissue significantly reduced the presence of adhesions in the colon and adjacent organs and decreased the activity of myeloperoxidase, a marker of neutrophil infiltration in the injured mucosa. The results suggest that cell therapy with adipose tissue can promote and/or accelerate the regeneration of damaged intestinal mucosa. It is concluded that the presence of adhesions and the determination of myeloperoxidase activity provide indications that adipose tissue can promote and/or accelerate the regeneration of inflammatory bowel mucosa. Universidade de São Paulo Institute of Biomedical Sciences Universidade Estadual Paulista Biosciences Institute Department of Pharmacology Universidade Paulista Institute of Health Sciences Universidade Federal da Grande Dourados School of Biological and Environmental Sciences Universidade Estadual Paulista Department of Biological Sciences Universidade Estadual Paulista Biosciences Institute Department of Pharmacology Universidade Estadual Paulista Department of Biological Sciences |
| Databáze: | OpenAIRE |
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