The light spot test: Measuring anxiety in mice in an automated home-cage environment
| Autor: | Aarts, E., Maroteaux, G., Loos, M., Koopmans, B., Kovačević, J., Smit, A.B., Verhage, M., van de Sluis, S., van der Horst, G.T., Levelt, C.N. |
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| Přispěvatelé: | Netherlands Institute for Neuroscience (NIN), Functional Genomics, Center for Neurogenomics and Cognitive Research, Molecular and Cellular Neurobiology, Complex Trait Genetics, Neuroscience Campus Amsterdam - Brain Mechanisms in Health & Disease, Cognitive and Systems Neuroscience (SILS, FNWI), Human genetics, NCA - Brain mechanisms in health and disease, Erasmus MC other, General Practice, Cell biology |
| Rok vydání: | 2015 |
| Předmět: |
Predictive validity
Male Light spot Light Anxiety Motor Activity Inbred C57BL Laboratory Developmental psychology Automation Mice Behavioral Neuroscience Anti-Anxiety Agents medicine Animals Psychological testing Automation Laboratory Psychological Tests Diazepam Animal Equipment Design Housing Animal Test (assessment) Mice Inbred C57BL Housing Home cage medicine.symptom Aversive Stimulus Psychology Neuroscience Photic Stimulation |
| Zdroj: | Behavioural Brain Research, 294, 123-30. Elsevier B.V. Behavioural Brain Research, 294, 123-130. Elsevier Aarts, E, Maroteaux, G P, Loos, M, Koopmans, B, Kovacevic, J, Smit, A B, Verhage, M & van der Sluis, S 2015, ' The light spot test: Measuring anxiety in mice in an automated home-cage environment ', Behavioural Brain Research, vol. 294, pp. 123-130 . https://doi.org/10.1016/j.bbr.2015.06.011 Behavioural Brain Research, 294, 123. Elsevier |
| ISSN: | 0166-4328 |
| DOI: | 10.1016/j.bbr.2015.06.011 |
| Popis: | Behavioral tests of animals in a controlled experimental setting provide a valuable tool to advance understanding of genotype-phenotype relations, and to study the effects of genetic and environmental manipulations. To optimally benefit from the increasing numbers of genetically engineered mice, reliable high-throughput methods for comprehensive behavioral phenotyping of mice lines have become a necessity. Here, we describe the development and validation of an anxiety test, the light spot test, that allows for unsupervised, automated, high-throughput testing of mice in a home-cage system. This automated behavioral test circumvents bias introduced by pretest handling, and enables recording both baseline behavior and the behavioral test response over a prolonged period of time. We demonstrate that the light spot test induces a behavioral response in C57BL/6J mice. This behavior reverts to baseline when the aversive stimulus is switched off, and is blunted by treatment with the anxiolytic drug Diazepam, demonstrating predictive validity of the assay, and indicating that the observed behavioral response has a significant anxiety component. Also, we investigated the effectiveness of the light spot test as part of sequential testing for different behavioral aspects in the home-cage. Two learning tests, administered prior to the light spot test, affected the light spot test parameters. The light spot test is a novel, automated assay for anxiety-related high-throughput testing of mice in an automated home-cage environment, allowing for both comprehensive behavioral phenotyping of mice, and rapid screening of pharmacological compounds. (C) 2015 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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