A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome
| Autor: | Warren C. McNabb, Simon M. Loveday, Karl Fraser, Peter H. Janssen, Laura G. Raymond, Nicole C. Roy, Stefan J. Hill, Ian M. Sims, L. Payling |
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| Rok vydání: | 2021 |
| Předmět: |
Protocol (science)
General Immunology and Microbiology Cell Culture General Neuroscience Systems biology digestive oral and skin physiology Genomics Computational biology Biology General Biochemistry Genetics and Molecular Biology Gut microbiome Breath methane Breath testing Human gut Microbiome Clinical Protocol lcsh:Science (General) Ex vivo lcsh:Q1-390 Uncategorized |
| Zdroj: | STAR Protocols, Vol 2, Iss 1, Pp 100227-(2021) |
| ISSN: | 2666-1667 |
| DOI: | 10.1016/j.xpro.2020.100227 |
| Popis: | Summary: This protocol describes the application of breath testing and ex vivo fermentations to study the association between breath methane and the composition and functionality of the gut microbiome. The protocol provides a useful systems biology approach for studying the gut microbiome in humans, which combines standardized methods in human breath testing and fecal sampling. The model described is accessible and easy to repeat, but its relative simplicity means that it can deviate from human physiological conditions. |
| Databáze: | OpenAIRE |
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