Preferential expression of scores of functionally and evolutionarily diverse DNA and RNA-binding proteins during Oxytricha trifallax macronuclear development
| Autor: | Alan M. Zahler, Zachary T. Neeb, Daniel J. Hogan, Sol Katzman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Protozoan Proteins lcsh:Medicine Gene Expression RNA-binding protein Genome Biochemistry Polymerases Database and Informatics Methods Gene duplication Gene expression lcsh:Science Phylogeny Genetics Multidisciplinary biology Messenger RNA High-Throughput Nucleotide Sequencing RNA-Binding Proteins Nucleic acids RNA polymerase Sequence Analysis Research Article Bioinformatics DNA recombination DNA repair Research and Analysis Methods Evolution Molecular 03 medical and health sciences Protein Domains Sequence Motif Analysis Macronucleus DNA-binding proteins Animals Gene Oxytricha Biology and life sciences Gene Expression Profiling lcsh:R RNA Proteins DNA DNA Protozoan biology.organism_classification 030104 developmental biology Gene Expression Regulation lcsh:Q Oxytricha trifallax Homologous recombination |
| Zdroj: | PLoS ONE PLoS ONE, Vol 12, Iss 2, p e0170870 (2017) |
| ISSN: | 1932-6203 |
| Popis: | During its sexual reproduction, the stichotrichous ciliate Oxytricha trifallax orchestrates a remarkable transformation of one of the newly formed germline micronuclear genomes. Hundreds of thousands of gene pieces are stitched together, excised from chromosomes, and replicated dozens of times to yield a functional somatic macronuclear genome composed of ~16,000 distinct DNA molecules that typically encode a single gene. Little is known about the proteins that carry out this process. We profiled mRNA expression as a function of macronuclear development and identified hundreds of mRNAs preferentially expressed at specific times during the program. We find that a disproportionate number of these mRNAs encode proteins that are involved in DNA and RNA functions. Many mRNAs preferentially expressed during macronuclear development have paralogs that are either expressed constitutively or are expressed at different times during macronuclear development, including many components of the RNA polymerase II machinery and homologous recombination complexes. Hundreds of macronuclear development-specific genes encode proteins that are well-conserved among multicellular eukaryotes, including many with links to germline functions or development. Our work implicates dozens of DNA and RNA-binding proteins with diverse evolutionary trajectories in macronuclear development in O. trifallax. It suggests functional connections between the process of macronuclear development in unicellular ciliates and germline specialization and differentiation in multicellular organisms, and argues that gene duplication is a key source of evolutionary innovation in this process. |
| Databáze: | OpenAIRE |
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