DNA Damage and Repair of Head and Neck Cancer Cells after Radio- and Chemotherapy
| Autor: | Anita Zwierzchlejska, Ireneusz Majsterek, Pawel Rusin, Alina Morawiec-Bajda, Anna Walczak, Dariusz Pytel, Tomasz Sliwinński, Dariusz Kaczmarczyk, Paweł Garncarek, Jurek Olszewski, Krzysztof Kusmierczyk |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male DNA Repair Cell Survival DNA repair DNA damage Antineoplastic Agents General Biochemistry Genetics and Molecular Biology Carcinoma medicine Humans Neoplasm Metastasis Cisplatin Radiotherapy business.industry Cancer DNA Neoplasm Hydrogen Peroxide medicine.disease Head and neck squamous-cell carcinoma Comet assay Kinetics stomatognathic diseases Head and Neck Neoplasms Cancer cell Carcinoma Squamous Cell Cancer research Female Comet Assay business DNA Damage medicine.drug |
| Zdroj: | ResearcherID |
| ISSN: | 1865-7125 0939-5075 |
| DOI: | 10.1515/znc-2009-7-821 |
| Popis: | DNA repair is critical for successful chemo- and radiotherapy of human tumours, because their genotoxic sensitivity may vary in different types of cancer cells. In this study we have compared DNA damage and the efficiency of its repair after genotoxic treatment with hydrogen peroxide, cisplatin and γ-radiation of head and neck squamous cell carcinoma (HNSCC). Lymphocytes and tissue cells from biopsies of 37 cancer patients and 35 healthy donors as well as the HTB-43 larynx cancer cell line were employed. The cell sensitivity to genotoxic treatment was estimated by the MTT survival assay. The extent of DNA damage and efficiency of its repair was examined by the alkaline comet assay. Among the examined treatments, we found that HNSCC cells were the most sensitive to γ-radiation and displayed impaired DNA repair. In particular, DNA damage was repaired less effectively in cells from HNSCC metastasis than healthy controls. In conclusion, our results suggest that the different genotoxic sensitivity of HNSCC cells may depend on their DNA repair capacity what in turn may be connected with the effectiveness of head and neck cancer therapy. |
| Databáze: | OpenAIRE |
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