Neuron-specific enolase as a novel biomarker reflecting tuberculosis activity and treatment response
| Autor: | Hee-Jae Cha, Mann-Hong Jung, Tae Won Jang, Bong-Kwon Chun, Jee-Yeong Jeong, Sung-Jin Nam, Chulho Oak |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Pulmonology Biopsy Phosphopyruvate hydratase Antitubercular Agents Fluorescent Antibody Technique 0302 clinical medicine Diagnosis 030212 general & internal medicine Lung biology Response Middle Aged Predictive value Up-Regulation Treatment Outcome Biomarker (medicine) Original Article Female Adult Treatment response endocrine system Tuberculosis Adolescent 030106 microbiology Enolase Antigens Differentiation Myelomonocytic C-reactive protein 03 medical and health sciences Young Adult Antigens CD Predictive Value of Tests Republic of Korea medicine Humans Tuberculosis Pulmonary Aged Retrospective Studies business.industry Macrophages medicine.disease nervous system Tissue Array Analysis Immunology biology.protein business Biomarkers |
| Zdroj: | The Korean Journal of Internal Medicine |
| ISSN: | 2005-6648 1226-3303 |
| Popis: | Background/Aims: It is not clear which tests are indicative of the activity and severity of tuberculosis (TB). This study aimed to investigate the predictive value of neuron-specific enolase (NSE) and to determine the origin of NSE in TB patients. Methods: A single-center retrospective analysis was conducted on newly diagnosed TB patients between January and December 2010. Patients were categorized into one of two disease groups (focal segmental or extensive) based on chest X-ray. Pre- and post-treatment NSE concentrations were evaluated. To determine the origin of serum NSE concentration, NSE staining was compared with macrophage-specific CD68 staining in lung tissues and with a tissue microarray using immunohistochemistry and immunofluorescence. Results: A total of 60 newly diagnosed TB patients were analyzed. In TB patients, NSE serum concentration was significantly increased and NSE level decreased after treatment (p < 0.001). In proportion to serum high-sensitivity C-reactive protein concentration, the mean serum concentration of NSE in the extensive group (25.12 ng/mL) was significantly higher than that in the focal segmental group (20.23 ng/mL, p = 0.04). Immunohistochemical staining revealed a large number of macrophages that stained positively for both NSE and CD68 in TB tissues. In addition, NSE signals mostly co-localized with CD68 signals in the tissue microarray of TB patients. Conclusions: Our results suggest that NSE may be a practical parameter that can be used to monitor TB activity and treatment response. Elevated serum NSE level originates, at least in part, from macrophages in granulomatous lesions. |
| Databáze: | OpenAIRE |
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