Recombinant human calcineurin B inhibits orthotopic hepatocellular carcinoma xenograt growth in mice by promoting apoptosis
| Autor: | Zhi-Hui He, Lin Minge, Shu-Hong Tian, Guo-Hui Yi, Yan-Da Lu, Shaojiang Zheng, Hong-Hai Li, Li-Ping Zheng, Shen-Hong Gu, Li-Zhen Fu, Xiaodian Zhang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Cisplatin
mice lcsh:Arctic medicine. Tropical medicine medicine.diagnostic_test lcsh:RC955-962 Chemistry apoptosis hepatocellular carcinoma General Medicine Cell cycle medicine.disease Flow cytometry Calcineurin In vivo Apoptosis Hepatocellular carcinoma medicine Cancer research cell cycle calcineurin Cyclin B1 medicine.drug |
| Zdroj: | Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 14, Pp 32-40 (2019) |
| ISSN: | 1995-7645 |
| DOI: | 10.4103/1995-7645.271978 |
| Popis: | Objective: To explore the effects of recombinant human calcineurin B (rhCNB) on hepatocellular carcinoma in mice. Methods: An in vivo mouse model with hepatocellular carcinoma was established, and the mice were randomized into the rhCNB, positive control and vehicle treatments groups. Tumor growth was assessed via bioluminescence using a small animal imaging system. Relative tumor proliferation rate and tumor growth inhibition were calculated. The expression of p53 and caspase-9 proteins in tumors were detected by immunohistochemistry. In vitro, flow cytometry was used to quantify the cell-cycle stages and rate of apoptosis. Western blotting and quantitative real-time PCR assays were used to evaluate the effects of rhCNB on protein and gene expression of CDK1, cyclin B1, p53 and caspase-9. Results: rhCNB at the higher dose significantly reduced tumor growth in vivo and caused tumor cell apoptosis in vitro. The rhCNB at the higher dose was as effective as cisplatin, and was safer. Conclusions: rhCNB has potent pro-apoptotic effects on tumor cells in vivo and in vitro and is well tolerated in vivo. |
| Databáze: | OpenAIRE |
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