Primary 3D Culture of Human Thyroid Follicle-Like Structures in Platelet Lysate-Based Gel
| Autor: | T. E. Skachkova, A. P. Polyakov, Yu.D. Khesuani, Andrey Kaprin, I. K. Sviridova, N. S. Sergeeva, V. A. Kirsanova, Vladimir Mironov |
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| Rok vydání: | 2020 |
| Předmět: |
Blood Platelets
0301 basic medicine medicine.medical_treatment Primary Cell Culture Thyroid Gland Gene Expression Complex Mixtures Thyroglobulin General Biochemistry Genetics and Molecular Biology Gel based 03 medical and health sciences Follicle 0302 clinical medicine medicine Humans Cell Size Chemistry Thyroid General Medicine Molecular biology In vitro Culture Media Ki-67 Antigen 030104 developmental biology medicine.anatomical_structure Thyroid Epithelial Cells Thyroidectomy Functional activity Platelet lysate Human thyroid Gels Biomarkers 030217 neurology & neurosurgery |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 169:564-570 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-020-04930-6 |
| Popis: | The results of 3D culturing of human thyroid follicle-like structures in a gel based on platelet lysate at the gel-air interface are presented. During culturing up to 4 months, no new follicle-like structures were formed and none were destroyed. During the first 2 months, most follicle-like structures increased in size; then, their grown decelerated, but they retained viability. Ki-67+ cells were observed in the majority of follicle-like structures. Most of them produced thyroglobulin. Follicle-like structures get closer, the number of contacts between them increased, and cluster appeared. Thus, the developed 3D culturing system in a gel based on platelet lysate is an adequate approach for maintaining structure and functional activity of human follicle-like structures in vitro for at least 2 months. |
| Databáze: | OpenAIRE |
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