Characterization of Na+/H+ exchange in sheep rumen epithelial cells kept in primary culture
| Autor: | Péter Gálfi, G. Gǎbel, S. Neogrády, H. Martens |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
animal structures
Primary culture Rumen Sodium-Hydrogen Exchangers medicine.medical_treatment Biology Epithelium Pathology and Forensic Medicine Amiloride medicine High doses Animals Diuretics Cells Cultured Fetus Sheep General Veterinary Growth factor Epithelial Cells Hydrogen-Ion Concentration Molecular biology Trypsinization medicine.anatomical_structure Biochemistry Cell Division medicine.drug |
| Zdroj: | Zentralblatt fur Veterinarmedizin. Reihe A. 43(6) |
| ISSN: | 0514-7158 |
| Popis: | Summary The study aimed at testing whether Na+/H+ exchange activity is maintained during isolation and cultivation of rumen epithelial cells (REC) and whether Na+/H+ exchange is altered during proliferation and differentiation. REC were isolated from sheep rumen epithelium by fractional trypsinization and further cultivated for up to 21 days. Characteristics of Na+ uptake into cultured REC were the same as previously described for the Na+/H+ exchange in the intact rumen epithelium. Na+ uptake was increased by intracellular acidification and was inhibited by high doses of amiloride. Amiloride inhibited 3H-thymidine incorporation into REC at concentrations similar to those found for the inhibition of Na+ uptake. Fetal calf serum, a growth factor, stimulated amiloride-sensitive Na+ uptake. We therefore conclude that cultured rumen epithelial cells express Na+/H+ exchange and that the activity of the exchange is at least correlated to the intensity of proliferation. |
| Databáze: | OpenAIRE |
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