Yorkie controls tube length and apical barrier integrity during airway development
| Autor: | Dimitrios K. Papadopoulos, Christos Samakovlis, Kassiani Skouloudaki, Pavel Tomancak, Elisabeth Knust, Dilan Khalili, Vasilios Tsarouhas, Ioannis Christodoulou |
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| Rok vydání: | 2019 |
| Předmět: |
Embryo
Nonmammalian Epithelium Article 03 medical and health sciences 0302 clinical medicine Animals Drosophila Proteins Humans Research Articles Actin 030304 developmental biology Cell Nucleus 0303 health sciences biology fungi Cell Membrane Nuclear Proteins YAP-Signaling Proteins Embryo Cell Biology Organ Size respiratory system Apical membrane biology.organism_classification Embryonic stem cell Actins Extracellular Matrix Cell biology body regions Trachea Cross-Linking Reagents Drosophila melanogaster Mutation Tube length Trans-Activators Gases Airway 030217 neurology & neurosurgery Protein Binding |
| Zdroj: | The Journal of Cell Biology Skouloudaki, K, Christodoulou, I, Khalili, D, Tsarouhas, V, Samakovlis, C, Tomancak, P, Knust, E & Papadopoulos, D K 2019, ' Yorkie controls tube length and apical barrier integrity during airway development ', Journal of Cell Biology, vol. 218, no. 8, pp. 2762-2781 . https://doi.org/10.1083/jcb.201809121 |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.201809121 |
| Popis: | Skouloudaki et al. identify an alternative role of the transcriptional coactivator Yorkie (Yki) in controlling water impermeability and tube size of developing Drosophila airways. Tracheal impermeability is triggered by Yki-mediated transcriptional regulation of δ-aminolevulinate synthase (Alas), whereas tube elongation is controlled by binding of Yki to the actin-severing factor Twinstar. Epithelial organ size and shape depend on cell shape changes, cell–matrix communication, and apical membrane growth. The Drosophila melanogaster embryonic tracheal network is an excellent model to study these processes. Here, we show that the transcriptional coactivator of the Hippo pathway, Yorkie (YAP/TAZ in vertebrates), plays distinct roles in the developing Drosophila airways. Yorkie exerts a cytoplasmic function by binding Drosophila Twinstar, the orthologue of the vertebrate actin-severing protein Cofilin, to regulate F-actin levels and apical cell membrane size, which are required for proper tracheal tube elongation. Second, Yorkie controls water tightness of tracheal tubes by transcriptional regulation of the δ-aminolevulinate synthase gene (Alas). We conclude that Yorkie has a dual role in tracheal development to ensure proper tracheal growth and functionality. |
| Databáze: | OpenAIRE |
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