| Autor: |
Yugang Huang, Jijun Fu, Yi Zhou, Xiaoya Zhao, Songjing Zhong, Yinhui Wei, He Wang, Lingran Du, Xin Li, Jing Zhao, Xing Chen, Guodong Ye, Xinlin Jiang, Junyang Zhong |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
ACS macro letters. 10(7) |
| ISSN: |
2161-1653 |
| Popis: |
Preventing endosomal entrapment of gene/vector nanocomplexes (NCs) remains a challenge for highly effective siRNA delivery. To address this problem, guanidinylated cyclic synthetic polypeptides (GCSPs) were synthesized using an efficient and easy method. GCSPs can condense siRNAs into NCs with an encapsulation efficiency of approximately 90%, over twice the effectiveness of Lipofectamine2000 (Lipo2000). The NCs can also mediate luciferase knockdown in HeLa cells with a silencing efficiency of 80%, nearly 2- and 1.1-fold that of Lipo2000 and PEI, respectively. More importantly, the NCs can enter cells by mimicking the bioactivity of cell-penetrating peptides (CPPs). NCs can also exert a nuclear localized function similar to nuclear localization signal peptides (NLSPs). Both biofunctions are helpful for preventing the common endosomal entrapment of NCs and greatly enhance the efficiency of siRNA delivery. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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