N-ras oncogene expression changes the growth characteristics of human melanoma in two independent SCID-hu mouse models
Autor: | Klaus Wolff, Sue A. Inoue, Burkhard Jansen, Hans-Georg Eichler, Hubert Pehamberger, Andrea van Elsas, Hermine Wadl, Peter I. Schrier |
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Rok vydání: | 1996 |
Předmět: |
Cancer Research
Xenotransplantation medicine.medical_treatment Transplantation Heterologous Gene Expression Mice SCID Oncogene Protein p21(ras) Biology medicine.disease_cause Mice Gene expression medicine Animals Humans Melanoma Gene Genetics Mutation Oncogene Point mutation medicine.disease Phenotype Genes ras Oncology Cancer research Cell Division |
Zdroj: | International Journal of Cancer. 67:821-825 |
ISSN: | 1097-0215 0020-7136 |
Popis: | Fifteen percent of all human melanomas carry mutations in ras genes, the majority of which are located in codon 61 of the N-ras gene. However, the biological significance of these mutations is as yet unknown. In this study, we investigated the influence of N-ras oncogene products mutated in codon 61 on the growth characteristics of human melanoma in vivo by establishing 2 SCID-hu mouse xenotransplantation models. Tumors grown in SCID mice injected with human melanoma carrying activated N-ras genes were significantly larger (p < 0.004) than tumors grown in animals injected with the appropriate control transfectants. Additionally, tumors with N-ras point mutations clearly showed a more pleomorphic phenotype than the control groups. Our results, obtained in 2 independent SCID-hu xenotransplantation models, suggest that mutated N-ras oncogene expression may be an important factor influencing growth characteristics of human melanoma without altering metastatic potential. These novel in vivo model systems provide a tool for further study of the biology of mutated ras in melanoma and should also prove useful for testing new and improved treatment strategies for human melanoma carrying mutated ras genes. |
Databáze: | OpenAIRE |
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