Antimicrobial Electrospun Polycaprolactone-Based Wound Dressings: An In Vitro Study About the Importance of the Direct Contact to Elicit Bactericidal Activity
| Autor: | Sofía Salido, Silvia Irusta, Manuel Arruebo, Gracia Mendoza, Enrique Gamez |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
integumentary system technology industry and agriculture equipment and supplies Critical Care and Intensive Care Medicine Antimicrobial Technology Advances Electrospinning 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine chemistry Polycaprolactone Emergency Medicine In vitro study Carvacrol Food science human activities Thymol |
| Zdroj: | Advances in Wound Care. 8:438-451 |
| ISSN: | 2162-1934 2162-1918 |
| DOI: | 10.1089/wound.2018.0893 |
| Popis: | Objective: To prepare efficient antibacterial carvacrol (CAR) and thymol (THY)-loaded electrospun polycaprolactone (PCL)-based wound dressings. Approach: Using electrospinning we were able to prepare wound dressings with antimicrobial action thanks to their large surface per volume ratio, which allows their loading with therapeutic amounts of active principles. By nuclear magnetic resonance we demonstrated that the antimicrobial compounds are donors of hydrogen bonds to the ester functional group in PCL, which acts as acceptor and that intermolecular interaction is responsible for the high drug loading achieved. Results: Those mats loaded with CAR and THY without the use of solubilizing agents were able to completely eradicate both Gram-positive (Staphylococcus aureus ATCC 25923) and Gram-negative (Escherichia coli S17 strain) bacteria at doses inferior to the ones needed when using the free nonsupported compounds. A superior antimicrobial action was observed for THY and CAR against Gram-negative bacteria than against Gram-positive bacteria, despite the higher hydrophilicity of the outer layer of Gram-negative bacteria. Innovation: We demonstrate that a direct contact between the bacteria and the dressing is required to elicit antimicrobial action. We also evaluated drug loadings by gas chromatography coupled with mass spectrometry and nuclear magnetic resonance validating a new analytical approach. Finally we were able to visualize the pathogenic bacteria on the dressings by confocal microscopy. Conclusion: The interaction between the PCL-based mat and the pathogenic bacteria is a key issue to achieve complete pathogen eradication. Under no-contact conditions, released CAR or THY from the electrospun mats did not exert any antimicrobial action at the doses tested. |
| Databáze: | OpenAIRE |
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