The inhibitory effect of glucose on phosphoenolpyruvate carboxykinase gene expression in cultured hepatocytes is transcriptional and requires glucose metabolism

Autor:	Fabienne Cournarie, Pascal Ferré, Dalila Azzout-Marniche, Marc Foretz, Fabienne Foufelle, Colette Guichard
Přispěvatelé:	Physiologie de la Nutrition et du Comportement Alimentaire (PNCA), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Université Paris Descartes - Paris 5 (UPD5)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), FORETZ, Marc
Jazyk:	angličtina
Rok vydání:	1999
Předmět:	Transcription Genetic endocrine system diseases [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Biochemistry chemistry.chemical_compound 0302 clinical medicine Structural Biology Gene expression [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] Glucose homeostasis MESH: Animals Promoter Regions Genetic [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Cells Cultured [SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism chemistry.chemical_classification 0303 health sciences MESH: Phosphoenolpyruvate Carboxykinase (GTP) MESH: Gene Expression Regulation Enzymologic [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular Networks [q-bio.MN] [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] MESH: Glucose Phosphoenolpyruvate carboxykinase Liver Female Phosphoenolpyruvate Carboxykinase (GTP) Transcription hormones hormone substitutes and hormone antagonists MESH: Cells Cultured endocrine system MESH: Rats Biophysics Carbohydrate metabolism Biology digestive system Gene Expression Regulation Enzymologic 03 medical and health sciences MESH: Promoter Regions Genetic Genetics [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] Animals Glucose-6-phosphate RNA Messenger Rats Wistar [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Molecular Biology 030304 developmental biology MESH: RNA Messenger Glucokinase MESH: Transcription Genetic nutritional and metabolic diseases [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Cell Biology MESH: Rats Wistar Rats Glucose Enzyme Glucose 6-phosphate chemistry Gluconeogenesis MESH: Female [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition 030217 neurology & neurosurgery MESH: Liver
Zdroj:	FEBS Letters FEBS Letters, Wiley, 1999, pp.527-32 FEBS Letters, 1999, 460 (3), pp.527-532. ⟨10.1016/s0014-5793(99)01407-6⟩ FEBS Letters, Wiley, 1999, 460 (3), pp.527-532. ⟨10.1016/s0014-5793(99)01407-6⟩
ISSN:	0014-5793 1873-3468
DOI:	10.1016/s0014-5793(99)01407-6⟩
Popis:	International audience; Phosphoenolpyruvate carboxykinase (PEPCK) is the rate-limiting enzyme of gluconeogenesis in the liver. PEPCK gene expression is controlled at the transcriptional level and is mainly regulated by hormones that are involved in glucose homeostasis. In this study, we have investigated the role of glucose on PEPCK gene expression in cultured hepatocytes. We demonstrate that glucose counteracts the stimulatory effect of glucocorticoids and cAMP on PEPCK expression. Glucose must be metabolized through glucokinase to have its inhibitory effect. The effect of glucose is mainly transcriptional and the region responsible for glucose inhibition is localized in the first 490 bp of the promoter.
Databáze:	OpenAIRE
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