Effect of melatonin on testicular ischemia/reperfusion injury in rats: is this effect related to the proinflammatory cytokines?
| Autor: | Fusun Baba, Hatice Ozbilge, Zehra Kurcer, Necla Celik, Nurten Aksoy, Elif Oguz |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
endocrine system medicine.medical_specialty medicine.medical_treatment Interleukin-1beta Ischemia Protein oxidation Antioxidants Proinflammatory cytokine Placebos Protein Carbonylation Melatonin chemistry.chemical_compound Malondialdehyde Internal medicine Testis medicine Animals Rats Wistar Peroxidase biology Interleukin-6 Tumor Necrosis Factor-alpha business.industry Obstetrics and Gynecology medicine.disease Rats Endocrinology Cytokine Reproductive Medicine chemistry Cytoprotection Reperfusion Injury Myeloperoxidase Immunology biology.protein Cytokines Inflammation Mediators business Reperfusion injury hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Fertility and Sterility. 89:1468-1473 |
| ISSN: | 0015-0282 |
| Popis: | Objective To determine whether the protective effect of melatonin in testicular ischemia/reperfusion (IR) injury is mediated by the proinflammatory molecules. Design Experimental study. Setting University pharmacology laboratory. Animal(s) Fifty-six 8-week-old male Wistar albino rats. Intervention(s) Left testicular artery and vein was occluded for 1 hour, followed by 3 hours or 24 hours of reperfusion. Melatonin (10 mg/kg IP) or vehicle (1% ethanol in saline) was given 10 minutes before ischemia. Main Outcome Measure(s) Malondialdehyde (MDA), protein carbonyl (PC) content, myeloperoxidase (MPO) activity, and proinflammatory cytokines TNF-α, IL-1β, and IL-6 were examined in testicular tissue after 3 hours of reperfusion. Histologic examination was made after 24 hours of reperfusion. Result(s) The MDA, PC, and MPO levels in testicular tissue increased significantly after IR, but the proinflammatory cytokine levels did not change. Melatonin treatment decreased lipid and protein oxidation and ameloriated histopathologic alterations induced by IR without any change in proinflammatory cytokine levels. Conclusion(s) The protective effect of melatonin on IR-induced testiculary injury is related to its antioxidant properties but not to proinflammatory cytokines. |
| Databáze: | OpenAIRE |
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