Cloning and characterization of a pair of novel genes that regulate production of extracellular enzymes in Bacillus subtilis
| Autor: | A. S.-H. Pang, S. Nathoo, Sui-Lam Wong |
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| Rok vydání: | 1991 |
| Předmět: |
Transcription
Genetic Operon Molecular Sequence Data Restriction Mapping Bacillus subtilis Biology Microbiology Restriction map Plasmid Gene expression Endopeptidases Amino Acid Sequence Cloning Molecular Promoter Regions Genetic Molecular Biology Gene Regulator gene Genetics Spores Bacterial Terminator Regions Genetic Base Sequence Levansucrase biology.organism_classification beta-Galactosidase Kinetics Biochemistry Genes Bacterial Protein Biosynthesis Plasmids Research Article |
| Zdroj: | Journal of bacteriology. 173(1) |
| ISSN: | 0021-9193 |
| Popis: | Two novel Bacillus subtilis genes that regulate the production of several extracellular enzymes were clones and characterized. These two genes are organized as part of an operon. When cloned in a multicopy plasmid, the first gene (tenA, transcription enhancement) stimulates alkaline protease production at the transcriptional level. The second gene (tenI) exerts an opposite effect to reduce alkaline protease production. The production of neutral protease, levansucrase, and alkaline protease can be stimulated up to 11- to 55-fold. Thus, tenA is a new member of the deg (regulatory genes for degradative enzymes) family in B. subtilis. A functional degS product is required to observe the stimulatory effect from tenA. Between the promoter and the ribosome-binding site of tenA, there exists a terminatorlike structure. Deletion of this structure doubles the expression of tenA. Neither tenA nor tenI is essential for cell growth and the production of extracellular enzymes. However, inactivation of these genes causes a delay in sporulation. This operon is located close to tre on the genetic linkage map. The overall organization of this operon and its relationship with other known regulatory factors in the deg family are discussed. |
| Databáze: | OpenAIRE |
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