A Two-Immunoglobulin-Domain Transmembrane Protein Mediates an Epidermal-Neuronal Interaction to Maintain Synapse Density
| Autor: | Salvatore J. Cherra, Yishi Jin |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
1.1 Normal biological development and functioning Neuroscience(all) Molecular Sequence Data Immunoglobulins Genetically Modified Biology Inhibitory postsynaptic potential Animals Genetically Modified Synapse 03 medical and health sciences Excitatory synapse Underpinning research Animals Humans Psychology Cholinergic synapse Amino Acid Sequence Caenorhabditis elegans Caenorhabditis elegans Proteins Neurons Neurology & Neurosurgery Epidermis (botany) General Neuroscience Neurosciences Cell biology 030104 developmental biology HEK293 Cells Silent synapse Synapses Neurological Cholinergic GABAergic Cognitive Sciences Epidermis Neuroscience |
| Zdroj: | Neuron, vol 89, iss 2 |
| ISSN: | 0896-6273 |
| DOI: | 10.1016/j.neuron.2015.12.024 |
| Popis: | Synaptic maintenance is essential for neural circuit function. In the C. elegans locomotor circuit, motor neurons are in direct contact with the epidermis. Here, we reveal a novel epidermal-neuronal interaction mediated by a two-immunoglobulin domain transmembrane protein, ZIG-10, that is necessary for maintaining cholinergic synapse density. ZIG-10 is localized at the cell surface of epidermis and cholinergic motor neurons, with high levels at areas adjacent to synapses. Loss of zig-10 increases the number of cholinergic excitatory synapses and exacerbates convulsion behavior in a seizure model. Mis-expression of zig-10 in GABAergic inhibitory neurons reduces GABAergic synapse number, dependent on the presence of ZIG-10 in the epidermis. Furthermore, ZIG-10 interacts with the tyrosine kinase SRC-2 to regulate the phagocytic activity of the epidermis to restrict cholinergic synapse number. Our studies demonstrate the highly specific roles of non-neuronal cells in modulating neural circuit function, through neuron-type-specific maintenance of synapse density. |
| Databáze: | OpenAIRE |
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