Stimulation of DNA Synthesis in Pancreatic Duct Cells by Gastrointestinal Hormones
| Autor: | Dante G. Scarpelli, Susan C. Hubchak, Mario M. Mangino |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism medicine.medical_treatment Vasoactive intestinal peptide Biology Secretin Gastrointestinal Hormones chemistry.chemical_compound Endocrinology Epidermal growth factor Cricetinae Internal medicine Internal Medicine medicine Animals Drug Interactions Growth Substances Cells Cultured Gastrin Mesocricetus Hepatology Growth factor Pancreatic Ducts Bombesin Membranes Artificial DNA medicine.anatomical_structure Gastrointestinal hormone chemistry Collagen Pancreas Gels hormones hormone substitutes and hormone antagonists Thymidine |
| Zdroj: | Pancreas. 7:271-279 |
| ISSN: | 0885-3177 |
| DOI: | 10.1097/00006676-199205000-00002 |
| Popis: | Pancreatic duct cells of the Syrian hamster were grown as monolayers on thin layers of type I collagen coated onto microporous membranes. The effects of a number of potential trophic factors were tested by their ability to increase [3H]thymidine incorporation into cellular DNA. To measure the effect of growth factors, cells were subjected to a period of growth factor depletion to induce a state of partial quiescence in DNA synthesis. Cells responded with a significant increase in thymidine incorporation after the addition of epidermal growth factor (EGF) alone or a growth factor mixture containing EGF plus insulin, transferrin, selenium, linoleic acid, bovine pituitary extract, triiodothyronine, and dexamethasone. When the serum substitute, Nu Serum IV (5%, vol/vol), was added to this mixture, addition of several gastrointestinal (GI) hormones including secretin, vasoactive intestinal polypeptide (VIP), bombesin, and gastrin caused significant increases in thymidine incorporation at concentrations of 0.01-1 microM. At 1 microM, these hormones stimulated DNA synthesis relative to their respective control in the order secretin (178%) greater than bombesin (153%) greater than VIP (138%) greater than gastrin (126%). Cholecystokinin octapeptide, a known trophic factor for pancreatic acinar cells, did not cause significant increases in thymidine incorporation in cultured duct cells. These results suggest that pancreatic duct cells possess receptors for a number of GI hormones and respond to the trophic effects of hormones known to stimulate pancreatic growth in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|