Evaluation of alkali and thermotolerant lipase from an indigenous isolated Bacillus strain for detergent formulation
| Autor: | Indu Bhushan, Srinivas Sistla, Vijeshwar Verma, Rashmi Saraswat |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:Biotechnology Metal ions in aqueous solution Environmental pollution Bacillus subtilis 01 natural sciences Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences chemistry.chemical_compound Pulmonary surfactant lcsh:TP248.13-248.65 010608 biotechnology Grease Food science Lipase lcsh:QH301-705.5 biology Strain (chemistry) Phosphate biology.organism_classification 030104 developmental biology lcsh:Biology (General) chemistry biology.protein Biotechnology |
| Zdroj: | Electronic Journal of Biotechnology, Vol 30, Iss, Pp 33-38 (2017) |
| ISSN: | 0717-3458 |
| DOI: | 10.1016/j.ejbt.2017.08.007 |
| Popis: | Background: Lipases are used in detergent industries to minimise the use of phosphate-based chemicals in detergent formulations. The use of lipase in household laundry reduces environmental pollution and enhances the ability of detergent to remove tough oil or grease stains. Results: A lipase-producing indigenous Bacillus subtilis strain [accession no. KT985358] was isolated from the foothills of Trikuta mountain in Jammu and Kashmir, India. The lipase (BSK-L) produced by this strain expressed alkali and thermotolerance. Lipase has an optimal activity at pH 8.0 and temperature 37°C, whereas it is stable at pH 6.0–9.0 and showed active lipolytic activity at temperatures 30 to 60°C. Furthermore, lipase activity was found to be stimulated in the presence of the metal ions Mn 2+ , K + , Zn 2+ , Fe 2+ and Ca 2+ . This lipase was resistant to surfactants, oxidising agents and commercial detergents, suggesting it as a potential candidate for detergent formulation. BSK-L displayed noticeable capability to remove oil stains when used in different washing solutions containing buffer, lipase and commercial detergent. The maximum olive oil removal percentage obtained was 68% when the optimum detergent concentration (Fena) was 0.3%. The oil removal percentage from olive oil-soiled cotton fabric increased with 40 U/mL of lipase. Conclusions: This BSK-L enzyme has the potential for removing oil stains by developing a pre-soaked solution for detergent formulation and was compatible with surfactants, oxidising agents and commercial detergents. Normal 0 false false false ES-CL X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:8.0pt; mso-para-margin-left:0cm; line-height:107%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-ansi-language:ES-CL;} Normal 0 false false false ES-CL X-NONE X-NONE |
| Databáze: | OpenAIRE |
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