Doxycycline down-regulates matrix metalloproteinase expression and inhibits NF-κB signaling in LPS-induced PC3 cells
| Autor: | Ceren Gonen Korkmaz, Deniz Ogut, Mehmet Zuhuri Arun, Serap Cilaker Micili, Bekir Ugur Ergur, Buket Reel |
|---|---|
| Přispěvatelé: | Ege Üniversitesi |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Male Lipopolysaccharide Angiogenesis IκB kinase Matrix metalloproteinase Western blotting chemistry.chemical_compound 0302 clinical medicine NF-KappaB Inhibitor alpha Cell Movement Doxycycline NF-kappa B General Medicine zymography prostate cancer Immunohistochemistry I-kappa B Kinase Blot ComputingMilieux_MANAGEMENTOFCOMPUTINGANDINFORMATIONSYSTEMS Gelatinases 030220 oncology & carcinogenesis MMPs InformationSystems_MISCELLANEOUS medicine.symptom medicine.drug Signal Transduction Histology PC3 cells Down-Regulation Inflammation Biology Pathology and Forensic Medicine 03 medical and health sciences Cell Line Tumor medicine Humans Neoplasm Invasiveness Cell Proliferation ComputerSystemsOrganization_COMPUTER-COMMUNICATIONNETWORKS Transcription Factor RelA Prostatic Neoplasms Molecular biology Matrix Metalloproteinases Enzyme Activation ComputingMethodologies_PATTERNRECOGNITION 030104 developmental biology chemistry NF-?B Cancer cell |
| Zdroj: | Folia histochemica et cytobiologica. 54(4) |
| ISSN: | 1897-5631 |
| Popis: | WOS: 000392718900001 PubMed ID: 27966209 Introduction. Matrix metalloproteinase enzymes (MMPs) play important role in inflammation, malignant cell proliferation, invasion and angiogenesis by mediating extracellular matrix degradation. Doxycycline, a synthetic tetracycline, behaves as a MMP inhibitor at a subantimicrobial dose and inhibits tumor cell proliferation, invasion and angiogenesis. The aberrant activity of nuclear factor kappa B (NF-kappa B) causes activation of MMPs and thereby proliferation and invasion of cancer cells. The aim of this study was to investigate the effects of doxycycline on the expression of MMPs in lipopolysaccharide (LPS)-induced PC3 human prostate cancer cells and the possible role of NF-kappa B signaling. Material and methods. PC3 cells were incubated with LPS (0.5 mu g/mL) for 24 h in the presence or absence of doxycycline (5 mu g/mL). The effects of LPS and doxycycline on the expressions of MMP-2, MMP-8, MMP-9, MMP-10, NF-kappa B/p65, I kappa B-alpha, p-I kappa B-alpha, IKK-beta were examined by Western blotting and immunohistochemistry in PC3 cells. Furthermore, relative proteinase activities of MMP-2 and MMP-9 were determined by gelatin zymography. Results. LPS increased expression and activity of MMP-9 and expression of MMP-8, MMP-10, NF-kappa B/p65, p-I kappa B-a, IKK-beta and doxycycline down-regulated its effects with the exception of MMP-10 expression. The expression of MMP-2 and I kappa B-a was affected by neither LPS nor doxycycline. Conclusions. Our findings indicate that doxycycline inhibits the expression of various MMPs and NF-kappa B signaling may play a role in the regulation of MMPs expression in LPS-induced PC3 human prostate cancer cells. Scientific Research Foundation of Ege University, Izmir, TurkeyEge University [12/ECZ/003] This research was supported by the Scientific Research Foundation of Ege University, Izmir, Turkey [12/ECZ/003 to Buket Reel]. |
| Databáze: | OpenAIRE |
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