Novel Amidohydrolytic Reactions in Oxidative Pyrimidine Metabolism: Analysis of the Barbiturase Reaction and Discovery of a Novel Enzyme, Ureidomalonase
| Autor: | Sakayu Shimizu, Jun Ogawa, Chee-Leong Soong |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
chemistry.chemical_classification
Barbituric acid Stereochemistry Hydrolysis Biophysics Cell Biology Oxidative phosphorylation Malonic acid Amides Biochemistry Amidohydrolases chemistry.chemical_compound Pyrimidines Enzyme chemistry Enzyme Induction Barbiturase Pyrimidine metabolism Urea Rhodococcus Organic chemistry Oxidation-Reduction Molecular Biology |
| Zdroj: | Biochemical and Biophysical Research Communications. 286:222-226 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.2001.5356 |
| Popis: | Amidohydrolytic reactions in oxidative pyrimidine metabolism were investigated in detail. Barbiturase has been reported to catalyze the amidohydrolysis of barbituric acid to urea and malonic acid. However, purification of the enzyme revealed that it catalyzes the ring-opening of barbituric acid to ureidomalonic acid. The existence of a consecutive enzyme named ureidomalonase, which hydrolyzes ureidomalonic acid to urea and malonic acid, was also discovered during the purification of barbiturase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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