Hesperidin protects against cadmium-induced pancreatitis by modulating insulin secretion, redox imbalance and iNOS/NF-ĸB signaling in rats

Autor: Onyedika G. Ani, Nwogo Ajuka Obasi, Kester U. Udeh, Felix E. Nwite, Friday I. Izekwe, Boniface A. Ale, Chinyere Aloke, E. U. Ekpono, P. M. Aja, Ademola C. Famurewa, I. O. Igwenyi, J.N. Awoke
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
medicine.medical_specialty
Nitric Oxide Synthase Type II
Protective Agents
medicine.disease_cause
030226 pharmacology & pharmacy
Antioxidants
General Biochemistry
Genetics and Molecular Biology
Superoxide dismutase
03 medical and health sciences
Hesperidin
chemistry.chemical_compound
0302 clinical medicine
Insulin-Secreting Cells
Malondialdehyde
Internal medicine
Insulin Secretion
medicine
Animals
Insulin
Rats
Wistar
General Pharmacology
Toxicology and Pharmaceutics
Inflammation
chemistry.chemical_classification
biology
Superoxide Dismutase
Glutathione peroxidase
NF-kappa B
General Medicine
Glutathione
Catalase
medicine.disease
Rats
Nitric oxide synthase
Oxidative Stress
030104 developmental biology
Endocrinology
Pancreatitis
chemistry
biology.protein
Oxidation-Reduction
Oxidative stress
Cadmium
Signal Transduction
Zdroj: Life Sciences. 259:118268
ISSN: 0024-3205
Popis: Aim Cadmium is a persistent ubiquitous environmental toxicant that elicits several biological defects on delicate body organs. Growing evidence suggests that cadmium (Cd) may perturb signaling pathways to induce oxidative pancreatitis. Thus, we explored whether hesperidin, a flavonone, could mitigate Cd-induced oxidative stress-mediated inflammation and pancreatitis in Wistar rats. Main methods Forty (40) rats randomly assigned to 5 groups (n = 8) were administered normal saline or hesperidin (Hsp) followed by Cd intoxication for 28 days. Key findings Cadmium accumulated in the pancreas of rats, and markedly decreased insulin, pancreatic superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities and glutathione (GSH) level. Cadmium considerably increased malondialdehyde (MDA), serum lipase and amylase activities. Cadmium induced pancreatic pro-inflammation via over-expression of inducible nitric oxide synthase (iNOS), nuclear factor-ĸB (NF-κB), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), along with histopathological alterations. Hesperidin prominently decreased serum amylase and lipase activities, and markedly increased insulin level, pancreatic antioxidant defense mechanism, whereas iNOS, NF-κB, IL-6 and TNF-α levels significantly decreased. Changes in histology confirmed our biochemical findings. Significance Our findings suggest that Cd induced pancreatitis via pro-inflammation and oxidative stress; Hsp, thus, protects against Cd-induced pancreatitis via attenuation of oxidative stress and proinflammatory responses in pancreas.
Databáze: OpenAIRE
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