Metabolomic Profile of Oviductal Extracellular Vesicles across the Estrous Cycle in Cattle
| Autor: | Marie Saint-Dizier, Sarah Janati Idrissi, Carmen Almiñana, Rustem Uzbekov, Julie Gatien, Ophélie Bernardi, Guillaume Tsikis, Pascal Salvetti, Pascal Mermillod, Daniel Le Bourhis |
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| Přispěvatelé: | Allice, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Université de Tours (UT), Faculty of Bioengineering and Bioinformatics, Moscow State University, Vetsuisse Faculty, Universität Zürich [Zürich] = University of Zurich (UZH), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Université de Tours, University of Zurich, Saint-Dizier, Marie |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
bovin oviduct Metabolite 1607 Spectroscopy Oviducts substrat énergétique chemistry.chemical_compound 0302 clinical medicine métabolite Biologie de la reproduction Spectroscopy media_common 2. Zero hunger Animal biology Principal Component Analysis Reproductive Biology fallopian tube 030219 obstetrics & reproductive medicine 630 Agriculture Chemistry [SDV.BA]Life Sciences [q-bio]/Animal biology résonance magnétique nucléaire Embryo General Medicine cycle œstral metabolomics Computer Science Applications acide aminé Metabolome Female 1606 Physical and Theoretical Chemistry extracellular vesicles métabolomique Ovulation endocrine system animal structures 1503 Catalysis energy substrates media_common.quotation_subject Estrous Cycle exosomes Luteal phase cellule de l'oviducte Catalysis Article Inorganic Chemistry Andrology 03 medical and health sciences Metabolomics Biologie animale 1312 Molecular Biology 1706 Computer Science Applications amino acids NMR Animals exosome Physical and Theoretical Chemistry Molecular Biology oviducte Estrous cycle 1604 Inorganic Chemistry urogenital system Organic Chemistry vésicule extracellulaire [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology Metabolism 10187 Department of Farm Animals Metabolic pathway 030104 developmental biology 570 Life sciences biology Cattle 1605 Organic Chemistry |
| Zdroj: | International Journal of Molecular Sciences Volume 20 Issue 24 International Journal of Molecular Sciences, MDPI, 2019, 20 (24), ⟨10.3390/ijms20246339⟩ International Journal of Molecular Sciences 24 (20), . (2019) |
| ISSN: | 1422-0067 1661-6596 |
| DOI: | 10.3390/ijms20246339 |
| Popis: | Oviductal extracellular vesicles (oEVs) have been proposed as key modulators of gamete/embryo maternal interactions. The aim of this study was to examine the metabolite content of oEVs and its regulation across the estrous cycle in cattle. Oviductal EVs were isolated from bovine oviducts ipsilateral and contralateral to ovulation at four stages of the estrous cycle (post-ovulatory stage, early and late luteal phases, and pre-ovulatory stage). The metabolomic profiling of EVs was performed by proton nuclear magnetic resonance spectroscopy (NMR). NMR identified 22 metabolites in oEVs, among which 15 were quantified. Lactate, myoinositol, and glycine were the most abundant metabolites throughout the estrous cycle. The side relative to ovulation had no effect on the oEVs&rsquo metabolite concentrations. However, levels of glucose-1-phosphate and maltose were greatly affected by the cycle stage, showing up to 100-fold higher levels at the luteal phase than at the peri-ovulatory phases. In contrast, levels of methionine were significantly higher at peri-ovulatory phases than at the late-luteal phase. Quantitative enrichment analyses of oEV-metabolites across the cycle evidenced several significantly regulated metabolic pathways related to sucrose, glucose, and lactose metabolism. This study provides the first metabolomic characterization of oEVs, increasing our understanding of the potential role of oEVs in promoting fertilization and early embryo development. |
| Databáze: | OpenAIRE |
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