Distinctive CD26 Expression on CD4 T-Cell Subsets
| Autor: | Rubén Varela-Calviño, Carlos Rafael-Vidal, Oscar J. Cordero, Samuel Garcia, Beatriz Malvar-Fernández, Juan E. Viñuela, Jose M Pego-Reigosa, Cristina Calviño-Sampedro |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
T cell
Dipeptidyl Peptidase 4 Cell T cell memory DPP4 Microbiology Biochemistry Article Memory T Cells Immune system Th2 Cells T-Lymphocyte Subsets Neoplasms medicine Humans Molecular Biology T helper polarization soluble CD26 Effector Chemistry Th1 Cells Phenotype QR1-502 Chimeric antigen receptor In vitro Cell biology medicine.anatomical_structure Gene Expression Regulation Leukocyte Common Antigens Th17 Cells Ex vivo |
| Zdroj: | Biomolecules Volume 11 Issue 10 Biomolecules, Vol 11, Iss 1446, p 1446 (2021) |
| ISSN: | 2218-273X |
| DOI: | 10.3390/biom11101446 |
| Popis: | Immune system CD4 T-cells with high cell-surface CD26 expression show anti-tumoral properties. When engineered with a chimeric antigen receptor (CAR), they incite strong responses against solid cancers. This subset was originally associated to human CD4 T helper cells bearing the CD45R0 effector/memory phenotype and later to Th17 cells. CD26 is also found in soluble form (sCD26) in several biological fluids, and its serum levels correlate with specific T cell subsets. However, the relationship between glycoprotein sCD26 and its dipeptidyl peptidase 4 (DPP4) enzymatic activity, and cell-surface CD26 expression is not well understood. We have studied ex vivo cell-surface CD26 and in vitro surface and intracellular CD26 expression and secretome’s sCD26 in cultured CD4 T cells under different polarization conditions. We show that most human CD26negative CD4 T cells in circulating lymphocytes are central memory (TCM) cells while CD26high expression is present in effector Th1, Th2, Th17, and TEM (effector memory) cells. However, there are significant percentages of Th1, Th2, Th17, and Th22 CD26 negative cells. This information may help to refine the research on CAR-Ts. The cell surface CD45R0 and CD26 levels in the different T helper subsets after in vitro polarization resemble those found ex vivo. In the secretomes of these cultures there was a significant amount of sCD26. However, in all polarizations, including Th1, the levels of sCD26 were lower (although not significantly) compared to the Th0 condition (activation without polarization). These differences could have an impact on the various physiological functions proposed for sCD26/DPP4. |
| Databáze: | OpenAIRE |
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