NIR Scaffold Bearing Three Handles for Biocompatible Sequential Click Installation of Multiple Functional Arms
| Autor: | Deju Ye, Yan Zhang, Yuqi Wang, Jianhui Weng, Jianguo Lin |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Scaffold
Biocompatible Materials Nanotechnology 010402 general chemistry 01 natural sciences Biochemistry Catalysis Mice chemistry.chemical_compound Colloid and Surface Chemistry Animals neoplasms chemistry.chemical_classification Spectroscopy Near-Infrared Biomolecule technology industry and agriculture General Chemistry equipment and supplies Biocompatible material Fluorescence 0104 chemical sciences surgical procedures operative chemistry Click chemistry Click Chemistry Azide |
| Zdroj: | Journal of the American Chemical Society. 142:2787-2794 |
| ISSN: | 1520-5126 0002-7863 |
| Popis: | Near-infrared (NIR) probes are ideal for fluorescence labeling and imaging of biological targets in living animals. However, the instability of common NIR dyes hampers the construction of NIR probes bearing multiple functional components such as biomolecules for specific targeting and imaging reagents for multimodality imaging. To overcome these limitations, we designed a novel NIR scaffold bearing two terminal alkynes as clickable handles and a chloride on the heptamethine backbone that allows nucleophilic substitution with an azide to generate the third clickable handle. This unique scaffold allows for facile installation of multiple functional arms for the construction of multifunctional NIR probes. Various biomacromolecules or imaging reagents can be introduced to the NIR scaffold by sequential one-pot click reactions under biocompatible conditions. The preclickable handle chloride on the NIR backbone does not interfere with the initial click reactions, and it can be easily transformed into an azide for a following click reaction. On the basis of this unique NIR scaffold, we developed a highly efficient method to construct diverse NIR probes containing multiple functional biomolecules including peptides, antibodies, nucleic acids, and NIR/PET (positron emission tomography) dual-modality imaging probes bearing tumor-targeting groups. NIR imaging or multimodality imaging using these probes was performed on live cells or tumor models on living mice. |
| Databáze: | OpenAIRE |
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