Functional expression in vitro of bovine visual rhodopsin
| Autor: | Sergey Zozulya, M.Yu. Natochin, Boris E. Shmukler, M.N. Garnovskaya, Shirokova Ep, Dumler Il, T.A. Zvyaga, Vsevolod V. Gurevich, Badalov Pr |
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| Rok vydání: | 1990 |
| Předmět: |
Rhodopsin
genetic structures Transcription Genetic Mutant Molecular Sequence Data Restriction Mapping Gene Expression Bioengineering Biochemistry Cell-free system Transcription (biology) Complementary DNA Gene expression Animals Cloning Molecular Site-directed mutagenesis Eye Proteins Molecular Biology biology Base Sequence Rod Opsins Phosphodiesterase DNA Molecular biology Recombinant Proteins Cell biology Genes Protein Biosynthesis Mutation biology.protein Cattle sense organs Retinal Pigments Biotechnology |
| Zdroj: | Protein engineering. 3(5) |
| ISSN: | 0269-2139 |
| Popis: | Expression of functionally active bovine visual rhodopsin was achieved by sequential transcription and translation in vitro of rhodopsin gene cDNA with co-translational insertion of the protein into phosphatidylcholine liposomes. The recombinant rhodopsin has functional, spectral and immunochemical properties similar to those of natural rhodopsin from bovine retina. Two mutant rhodopsins, Cys316----Ser and Asp330----Asn, Asp331----Asn, were produced by oligonucleotide-directed mutagenesis. The first mutation does not affect rhodopsin's ability to stimulate transducin GTPase and visual cGMP phosphodiesterase activities, while the second double mutation leads to a sharp decrease in rhodopsin activity. |
| Databáze: | OpenAIRE |
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