Metabolite profiling of small cerebrospinal fluid sample volumes with gas chromatography-mass spectrometry: Application to a rat model of multiple sclerosis
| Autor: | Bas Muilwijk, Ernst Suidgeest, M. Tienstra, Marek J. Noga, Theo M. Luider, I. Bobeldijk, H. van Aken, Amos Attali, Thomas Hankemeier, Tinka Tuinstra, Leon Coulier, Sabina Bijlsma |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Silylation
Endocrinology Diabetes and Metabolism Metabolite Clinical Biochemistry Mass spectrometry Biochemistry Vial Multiple sclerosis chemistry.chemical_compound Metabolomics Cerebrospinal fluid EELS - Earth Environmental and Life Sciences TNO Bedrijven medicine Animal model Chromatography EAE model Life Triskelion BV medicine.disease chemistry Health Gas chromatography–mass spectrometry GC-MS Healthy for Life QS - Quality & Safety Healthy Living |
| Zdroj: | Metabolomics, 1, 9, 78-87 Metabolomics, 9(1), 78-87 |
| Popis: | Analysis of metabolites in biofluids by gas chromatography-mass spectrometry (GC-MS) after oximation and silylation is a key method in metabolomics. The GC-MS method was modified by a modified vial design and sample work-up procedure in order to make the method applicable to small volumes of cerebrospinal fluid (CSF), i. e. 10 μL, with similar coverage compared to the standard procedure using ≥100 μL of CSF. The data quality of the modified GC-MS method was assessed by analyzing a study sample set in an animal model for multiple sclerosis, including repetitively analysed quality control rat CSF samples. Automated normalization and intra- and inter-batch correction significantly improved the data quality with the majority of metabolites showing a relative standard deviation |
| Databáze: | OpenAIRE |
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