Homologous and Homeologous Intermolecular Gene Conversion Are Not Differentially Affected by Mutations in the DNA Damage or the Mismatch Repair Genes RAD1, RAD50, RAD51, RAD52, RAD54, PMS1 and MSH2
| Autor: | Michael A. Resnick, Gregory Porter, Jim Westmoreland, Scott Priebe |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Recombination
Genetic Genetics Mitotic crossover Base Sequence DNA Repair FLP-FRT recombination Genes Fungal Molecular Sequence Data RAD51 Non-allelic homologous recombination Saccharomyces cerevisiae Investigations Biology Genetic recombination Molecular biology Non-homologous end joining Mutation Ectopic recombination Amino Acid Sequence Gene conversion DNA Fungal Sequence Alignment |
| Zdroj: | Genetics. 143:755-767 |
| ISSN: | 1943-2631 |
| DOI: | 10.1093/genetics/143.2.755 |
| Popis: | Mismatch repair (MMR) genes or genes involved in both DNA damage repair and homologous recombination might affect homeologous us. homologous recombination differentially. Spontaneous mitotic gene conversion between a chromosome and a homologous or homeologous donor sequence (14% diverged) on a single copy plasmid was examined in wild-type Saccharomyces cerevisiae strains and in MMR or DNA damage repair mutants. Homologous recombination in rad51, rad52 and rad54 mutants was considerably reduced, while there was little effect of radl, rad50, pms1 and msh2 null mutations. DNA divergence resulted in no differential effect on recombination rates in the wild type or the mutants; there was only a five- to 10-fold reduction in homeologous relative to homologous recombination regardless of background. Since DNA divergence is known to affect recombination in some systems, we propose that differences in the role of MMR depends on the mode of recombination and/or the level of divergence. Based on analysis of the recombination breakpoints, there is a minimum of three homologous bases required at a recombination junction. A comparison of Rad+ vs. rad52 strains revealed that while all conversion tracts are continuous, elimination of RAD52 leads to the appearance of a novel class of very short conversion tracts. |
| Databáze: | OpenAIRE |
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