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Objectives: Dysfunction of the thyroid gland has a negative effect on the male reproductive system. Studies also show that extracellular matrix (ECM) components play an essential role in testicular development and function. In hypothyroidism, there is a significant disruption in the ECM structure of mammalian tissues. In addition, notable changes have been reported in the germ cell population under a hypothyroid state. This study aimed to investigate the relationship between ECM proteins and apoptosis of testicular germ cells due to hypothyroidism. Materials and Methods: In the present experimental study, 20 male Balb/C mice were divided into control and hypothyroid groups. The hypothyroid group received 0.05% 6-n-propyl-2-thiouracil (PTU) through drinking water for 35 days. Finally, real-time polymerase chain reaction, immunohistochemistry, periodic acid-Schiff (PAS) staining, terminal transferase-mediated dUTP nick-end labelling (TUNEL) assay, and biochemical measurements were performed after hypothyroidism confirmation. Results: Laminin α5 and collagen IV mRNA levels were upregulated in the hypothyroid group compared to the controls (P |
