Hypoxemia induces expression of heme oxygenase-1 and heme oxygenase-2 proteins in the mouse myocardium
| Autor: | Kazumichi Furuyama, Yotaro Shinozawa, Kazunobu Ishikawa, Satoru Yokoyama, Fumiko Date, Shigeki Shibahara, Masao Ono, Kazuhisa Takeda, Feng Han |
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| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty Bilirubin Mice Nude Oxidative phosphorylation Biology Biochemistry Isozyme Hypoxemia Mice chemistry.chemical_compound Internal medicine medicine Animals Hypoxia Lung Molecular Biology Heme Myocardium Heart General Medicine Anatomy Hypoxia (medical) Mice Inbred C57BL Heme oxygenase Endocrinology medicine.anatomical_structure Liver chemistry Heme Oxygenase (Decyclizing) medicine.symptom Heme Oxygenase-1 |
| Zdroj: | Journal of Biochemistry. 147:143-151 |
| ISSN: | 0021-924X |
| Popis: | Heme oxygenase (HO) catalyzes oxidative breakdown of heme, and constitutes two isozymes, HO-1 and HO-2. Here, we explored the tissue-specific regulation of expression of HO-1 and HO-2 under hypoxemia. There was no significant change in the overall expression levels of HO-1 and HO-2 mRNAs and proteins in the lung during adaptation of C57BL/6 mice to normobaric hypoxia (10% O(2)). However, immunohistochemical analysis revealed the increased expression of HO-1 and HO-2 proteins after 28 days of normobaric hypoxia in the pulmonary venous myocardium that is the extension of the left atrial myocardium into pulmonary venous walls. Moreover, the expression of HO-2 protein was increased in the sub-endocardial myocardium of ventricles under hypoxia, while HO-1 protein level was increased in the full-thickness walls. Thus, hypoxemia induces expression of both HO-1 and HO-2 proteins in the myocardium. Using C57BL/6 mice lacking HO-2 (HO-2(-/-)), which manifest chronic hypoxemia, we also showed that the HO-1 protein level in the lung was similar between HO-2(-/-) mice and wild-type mice. Unexpectedly, HO-1 protein level was lower by 35% in the HO-2(-/-) mouse liver than the wild-type liver. These results indicate that the expression of HO-1 protein is regulated in a tissue-specific manner under hypoxemia. |
| Databáze: | OpenAIRE |
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