Molecular dosimetry of sister chromatid exchange induction in 9L cells treated with 6-thioguanine
| Autor: | W.J. Bodell |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Alkylating Agents
DNA damage Health Toxicology and Mutagenesis Sister chromatid exchange Biology Toxicology chemistry.chemical_compound Genetics Tumor Cells Cultured Sister chromatids Animals Thioguanine Metaphase Genetics (clinical) Dose-Response Relationship Drug Glioma Molecular biology Rats Dose–response relationship Nimustine Biochemistry chemistry Cell culture Ethylnitrosourea Sister Chromatid Exchange DNA DNA Damage |
| Zdroj: | Mutagenesis. 6(3) |
| ISSN: | 0267-8357 |
| Popis: | The induction of sister chromatid exchanges (SCE) in 9L cells treated with 6-thioguanine (6-TG) has been investigated. A 24 h treatment with 0.2 microM 6-TG induced approximately 28 SCE/metaphase. The dose-response curve was linear at doses below 0.2 microM and had a slope of 139 SCE/metaphase/microM 6-TG. At concentrations of 0.023 to 1 microM, incorporation of 6-TG in DNA was linear with dose. The slope of the dose-response curve was 4135 mumol 6-TG/mol DNA/microM 6-TG. Comparison of these results with those obtained in our previous studies of the monofunctional alkylating agent ethylnitrosourea and the bifunctional alkylating agent 3-(4-amino-2-methyl-5-pyrimidinyl)methyl-1-(2-chloroethyl)-1-nitrosourea suggest that to accurately estimate the effectiveness of particular DNA modifications at inducing SCE, the extent of formation of these DNA modifications must be known. |
| Databáze: | OpenAIRE |
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