A sensitive Enzyme-Linked Immunosorbent Assay (ELISA) against the major EBV-associated antigens. I. Correlation between ELISA and immunofluorescence titers using purified antigens
| Autor: | Janos Luka, Robert C. Chase, Gary R. Pearson |
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| Rok vydání: | 1984 |
| Předmět: |
Herpesvirus 4
Human medicine.drug_class viruses Immunology Nasopharyngeal neoplasm Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay medicine.disease_cause Monoclonal antibody Immunofluorescence Virus Immunoenzyme Techniques Viral Matrix Proteins Viral Proteins Antigen hemic and lymphatic diseases medicine Humans Immunology and Allergy Infectious Mononucleosis Antigens Viral biology medicine.diagnostic_test Chemistry Nasopharyngeal Neoplasms Epstein–Barr virus Virology Molecular biology Titer Epstein-Barr Virus Nuclear Antigens biology.protein Capsid Proteins Antibody |
| Zdroj: | Journal of Immunological Methods. 67:145-156 |
| ISSN: | 0022-1759 |
| Popis: | An enzyme-linked immunosorbent assay (ELISA) was developed for the major Epstein-Barr virus (EBV) associated antigens. The ELISA assay was based on purified protein components of virus-capsid antigen (VCA), early antigen (EA), membrane antigen (MA) and the nuclear antigen (EBNA). These antigens, except EBNA, were purified on immunoaffinity columns containing monoclonal antibodies, while EBNA was purified by biochemical methods. Screening of 69 human sera including 26 sera from individuals with infectious mononucleosis against these antigens in ELISA showed a good correlation with immunofluorescence titers against the major groups of EBV associated antigens. The results indicated that the ELISA technique using purified EBV proteins could be useful for detecting and monitoring antibody responses to different EBV proteins in patients with EBV-associated diseases. |
| Databáze: | OpenAIRE |
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