Simultaneous quantification of dexamethasone and 6β-hydroxydexamethasone in rabbit plasma, aqueous and vitreous humor, and retina by UHPLC-MS/MS
| Autor: | Jiang Wang, Sharron Stewart, Diaa Shakleya, Ashok Krishna, Lin Xu, Patrick J. Faustino, Yan Wang, Jianghong Gu |
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| Rok vydání: | 2021 |
| Předmět: |
Metabolite
Clinical Biochemistry Aqueous humor 01 natural sciences Uhplc ms ms Dexamethasone Retina Analytical Chemistry Aqueous Humor 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Tandem Mass Spectrometry medicine Animals General Pharmacology Toxicology and Pharmaceutics Chromatography High Pressure Liquid Aqueous solution Chromatography Chemistry 010401 analytical chemistry Rabbit (nuclear engineering) General Medicine 0104 chemical sciences Vitreous Body Medical Laboratory Technology medicine.anatomical_structure 030221 ophthalmology & optometry Rabbits Rabbit plasma medicine.drug |
| Zdroj: | Bioanalysis. 13(13) |
| ISSN: | 1757-6199 |
| Popis: | Aim: To develop and validate a fit for purpose method for the simultaneous determination of dexamethasone and its major metabolite, 6β-hydroxydexamethasone, in rabbit plasma and ocular matrices to measure the in vivo release and distribution profile of dexamethasone from intravitreal implants. Materials & methods: An UHPLC–MS/MS system was employed to perform the bioanalysis. The method was validated according to the US FDA Bioanalytical Method Validation Guidance for Industry. Results & conclusion: The method was found to be fit-for-purpose for the described biological matrices and had a LLOQ of 0.1 ng/ml. |
| Databáze: | OpenAIRE |
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