Urinary 17 alpha-hydroxyprogesterone in management of 21-hydroxylase deficiency
| Autor: | Y. J. Lim, Arthur B.W. Yong, G. L. Warne, J. Montalto |
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| Rok vydání: | 1995 |
| Předmět: |
Male
medicine.medical_specialty Pregnanetriol Chromatography Gas Urinary system Radioimmunoassay Enzyme-Linked Immunosorbent Assay Urine chemistry.chemical_compound Column chromatography Predictive Value of Tests Internal medicine medicine Hydroxyprogesterones Humans Congenital adrenal hyperplasia Child Glucocorticoids Morning biology Adrenal Hyperplasia Congenital business.industry 17-alpha-Hydroxyprogesterone 21-Hydroxylase Infant medicine.disease Circadian Rhythm Endocrinology chemistry Case-Control Studies Child Preschool Pediatrics Perinatology and Child Health biology.protein Female Gas chromatography business |
| Zdroj: | Journal of paediatrics and child health. 31(1) |
| ISSN: | 1034-4810 |
| Popis: | Objectives: The study was designed to assess the reliability of measurement of 24-hour urinary 17α-hydroxyprogesterone (17-OHP) by radio-immunoassay (RIA) as an alternative biochemical assessment for monitoring the treatment of congenital adrenal hyperplasia (CAH) due to 21 -hydroxylase deficiency (21 -OHD) and to assess the need for sample purification by column chromatography to improve assay specificity. Methodology: Morning serum 17-OHP was measured using RIA and 24-hour urinary pregnanetriol using gas chromatography. Twenty-four-hour urinary 17-OHP was measured in samples from 17 prepubertal patients with CAH due to 21 -OHD, and 20 normal prepubertal children as controls. In 24 urine samples, RIA of 17-OHP was performed with and without column chromatography. Results: There was a good correlation between 24-hour urinary 17-OHP and 24-hour urinary pregnanetriol (r = 0.962, P |
| Databáze: | OpenAIRE |
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