Profiling of specific long non-coding RNA signatures identifies ST8SIA6-AS1 AS a novel target for breast cancer
| Autor: | Yanling Feng, Zhanghan Chen, Jie Huang, Zehuan Li, Ying Jiang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Endogeny RNA-binding protein Breast Neoplasms Biology Heterogeneous-Nuclear Ribonucleoproteins Cell Line 03 medical and health sciences 0302 clinical medicine Breast cancer Cell Movement Drug Discovery Genetics medicine Biomarkers Tumor Humans Molecular Biology Gene Genetics (clinical) Cell Proliferation Neoplasm Staging Microarray analysis techniques Competing endogenous RNA RNA RNA-Binding Proteins medicine.disease Microarray Analysis Long non-coding RNA Sialyltransferases DNA-Binding Proteins Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Molecular Medicine Female RNA Long Noncoding |
| Zdroj: | The journal of gene medicineREFERENCES. 23(2) |
| ISSN: | 1521-2254 |
| Popis: | BACKGROUND Breast cancer is the most commonly diagnosed cancer among women and is also the leading cause of cancer death for which the treatment and methods of diagnosis remain unsatisfied. Long non-coding RNA (lncRNA) plays an important role in the occurrence and development of tumors, including breast cancer. We aimed to seek new and efficient treatment targets by analyzing the lncRNA expression profiles of breast cancer. METHODS A competitive endogenous RNA microarray was used to investigate the profiles of differentially expressed lncRNAs. Quantitative real-time polymerase chain reaction analysis (qRT-PCR) validated the top differentially expressed lncRNAs in 107 pairs of breast cancer tissues and adjacent normal tissues. cis- and trans-regulation mRNAs of lncRNAs were used to perform enrichment analysis. Cell function assays were used to explore the functions of ST8SIA6-AS1. RESULTS Seven lncRNAs, comprising ST8SIA6-AS1, lnc-HIST1H2BJ-5:1, lnc-PRICKLE2-3:2, RP1-86C11.7, RP11-15F12.1, ZNF670-ZNF695 and lnc-STRN3-12:1, were shown to be significantly up-regulated in breast cancer. lncRNA ST8SIA6-AS1 was associated with TNM staging and Ki-67 index. The cell function assays showed that ST8SIA6-AS1 can promote the proliferation, migration and invasion of breast cancer cells. The functions of ST8SIA6-AS1 were explored and the competing endogenous RNA mode showed that miR-4252 was a potential candidate. Its target genes were further predicted. The lncRNA-protein mode showed three potential candidate RNA binding proteins: NONO, QKI and RBMX. CONCLUSIONS lncRNA ST8SIA6-AS1 can promote the proliferation, migration and invasion of breast cancer cells. By hypothesizing two different functional modes of ST8SIA6-AS1, we found lncRNA ST8SIA6-AS1 may contribute to breast cancer progression through miR-4252 or interacting with RNA binding proteins: NONO, QKI and RBMX. |
| Databáze: | OpenAIRE |
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