Rapid high-performance liquid chromatographic method for the estimation of toxicological levels of 25-hydroxycholecalciferol (25-OH D3) in human serum

Autor:	Roman S. Lorenc, Jacek Łukaszkiewicz, Kazimierz Kozłowski
Rok vydání:	1983
Předmět:	Solvent system Vitamin Chromatography Extraction (chemistry) Toxicology High-performance liquid chromatography Hexane chemistry.chemical_compound chemistry Humans 25 hydroxycholecalciferol In patient Acetonitrile Chromatography High Pressure Liquid Calcifediol
Zdroj:	Journal of Applied Toxicology. 3:269-271
ISSN:	1099-1263 0260-437X
Popis:	A reversed-phase HPLC method for determination of 25-hydroxycholecalciferol (25-OH D3) in serum samples is described. The method involves extraction of 25-OH D3 with hexane, followed by differential resolubilization in acetonitrile. The sample is then applied directly on the C18 bonded phase column of the liquid chromatograph, and developed in a acetonitrile-water solvent system. In the range 12.5-400 ng, a linear relationship was observed between detector response and the amount of 25-OH D3 placed on the column. Also, a very good correlation was observed between the amounts of 25-OH D3 added to serum samples, and the calculated amounts. The method can be used for screening for toxicological levels of 25-OH D3 in patients treated with high doses of vitamin D3.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7cbe4bd45984ef9b05ef7048dff6c3a9 https://doi.org/10.1002/jat.2550030602 Zobrazit plný text záznamu Plný text