Long Pentraxin PTX3 Upregulates Tissue Factor Expression in Human Endothelial Cells
| Autor: | Giovanni de Gaetano, Roberto Lorenzet, Giuseppe Peri, Antonio Bastone, Angelomaria Di Santo, Maria Benedetta Donati, Alberto Mantovani, E. Napoleone |
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| Rok vydání: | 2002 |
| Předmět: |
Lipopolysaccharides
Umbilical Veins Endothelium Inflammation Cell Separation Biology Thromboplastin Proinflammatory cytokine Tissue factor NF-KappaB Inhibitor alpha medicine Humans RNA Messenger Antigens Blood Coagulation Cells Cultured Tumor Necrosis Factor-alpha NF-kappa B Transcription Factor RelA PTX3 Proto-Oncogene Proteins c-rel Up-Regulation DNA-Binding Proteins Endothelial stem cell Serum Amyloid P-Component IκBα C-Reactive Protein medicine.anatomical_structure Immunology Cancer research I-kappa B Proteins Tumor necrosis factor alpha Endothelium Vascular medicine.symptom Cardiology and Cardiovascular Medicine Dimerization Acute-Phase Proteins Interleukin-1 |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 22:782-787 |
| ISSN: | 1524-4636 1079-5642 |
| DOI: | 10.1161/01.atv.0000012282.39306.64 |
| Popis: | Inflammation is a major contributing factor to atherosclerotic plaque development and ischemic heart disease. PTX3 is a long pentraxin that was recently found to be increased in patients with acute myocardial infarction. Because tissue factor (TF), the in vivo trigger of blood coagulation, plays a dominant role in thrombus formation after plaque rupture, we tested the possibility that PTX3 could modulate TF expression. Human umbilical vein endothelial cells, incubated with endotoxin (lipopolysaccharide) or the inflammatory cytokines interleukin-1β and tumor necrosis factor-α, expressed TF. The presence of PTX3 increased TF activity and antigen severalfold in a dose-dependent fashion. PTX3 exerted its effect at the transcription level, inasmuch as the increased levels of TF mRNA, mediated by the stimuli, were enhanced in its presence. The increase in mRNA determined by PTX3 originated from an enhanced nuclear binding activity of the transacting factor c-Rel/p65, which was mediated by the agonists and measured by electrophoretic mobility shift assay. The mechanism underlying the increased c-Rel/p65 activity resided in an enhanced degradation of the c-Rel/p65 inhibitory protein IκBα. In the area of vascular injury, during the inflammatory response, cell-mediated fibrin deposition takes place. Our results suggest that PTX3, by increasing TF expression, potentially plays a role in thrombogenesis and ischemic vascular disease. |
| Databáze: | OpenAIRE |
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