STRA8-deficient spermatocytes initiate, but fail to complete, meiosis and undergo premature chromosome condensation
| Autor: | Pierre Chambon, Nadège Vernet, Christine Dennefeld, Norbert B. Ghyselinck, Mustapha Oulad-Abdelghani, Manuel Mark, Hugues Jacobs, Betty Féret, Carmen-Alina Codreanu |
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| Přispěvatelé: | Peney, Maité, Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institute of Medical Technology and Tampere University Hospital, University of Tampere [Finland], Institut Clinique de la Souris (ICS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Male
MESH: Chromosomes Mammalian MESH: Gene Targeting MESH: Spermatocytes S Phase Mice 0302 clinical medicine Spermatocytes Testis Meiotic S phase MESH: Animals MESH: Proteins Meiotic Prophase I 0303 health sciences [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] Synaptonemal Complex MESH: Testis MESH: S Phase [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] Meiosis MESH: Chromosome Pairing 030220 oncology & carcinogenesis Premature chromosome condensation Gene Targeting MESH: Spermatogenesis Premeiotic DNA replication Spo11 MESH: Mutation Biology MESH: Synaptonemal Complex 03 medical and health sciences Animals Spermatogenesis MESH: Metaphase Mitosis MESH: Mice Alleles Metaphase Adaptor Proteins Signal Transducing 030304 developmental biology MESH: Meiotic Prophase I MESH: Alleles Proteins Cell Biology Chromosomes Mammalian Molecular biology MESH: Male Chromosome Pairing MESH: Meiosis Mutation biology.protein |
| Zdroj: | Journal of Cell Science Journal of Cell Science, 2008, 121 (Pt 19), pp.3233-42. ⟨10.1242/jcs.035071⟩ Journal of Cell Science, Company of Biologists, 2008, 121 (Pt 19), pp.3233-42. ⟨10.1242/jcs.035071⟩ |
| ISSN: | 0021-9533 1477-9137 |
| DOI: | 10.1242/jcs.035071 |
| Popis: | International audience; We analysed the phenotypic outcome of a Stra8-null mutation on male meiosis. Because the mutant spermatocytes (1) underwent premeiotic DNA replication, (2) displayed cytological features attesting initiation of recombination and of axial-element assembly, and (3) expressed Spo11 and numerous other meiotic genes, it was concluded that STRA8 is dispensable for meiotic initiation. The few mutant spermatocytes that progressed beyond leptonema showed a prolonged bouquet-stage configuration, asynapsis and heterosynapsis, suggesting function(s) of STRA8 in chromosome pairing. Most importantly, a large number of mutant leptotene spermatocytes underwent premature chromosome condensation, within 24 hours following the meiotic S phase. This phenomenon yielded aberrant metaphase-like cells with 40 univalent chromosomes, similar to normal mitotic metaphases. From these latter observations and from the wild-type pattern of Stra8 expression, we propose that, in preleptotene spermatocytes, STRA8 is involved in the process that leads to stable commitment to the meiotic cell cycle. |
| Databáze: | OpenAIRE |
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