PAS-1, a protein affinity purified from Ascaris suum worms, maintains the ability to modulate the immune response to a bystander antigen
| Autor: | Claudia Affonso Silva Araujo, Mahasti Sahihi de Macedo, Telma M Oshiro, C. S. Enobe, M. F. Macedo-Soares |
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| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.drug_class Helminth protein Immunology Antibodies Helminth Dose-Response Relationship Immunologic Down-Regulation Immunoglobulin E Monoclonal antibody Immune tolerance Mice Immune system Th2 Cells Antigen medicine Immune Tolerance Immunology and Allergy Animals Ascaris suum Ascariasis Mice Inbred BALB C biology Cell Biology Bystander Effect Helminth Proteins Th1 Cells biology.organism_classification Molecular biology Interleukin-10 Ovalbumin Antigens Helminth Antibody Formation biology.protein Female Interleukin-4 |
| Zdroj: | Immunology and cell biology. 84(2) |
| ISSN: | 0818-9641 |
| Popis: | Helminth infections and parasite components have potent immunomodulatory effects on a host's immune system. In the present study, we investigated the effect of PAS-1, a protein component of Ascaris suum adult worms recognized by a monoclonal antibody (MAIP-1), on humoral and cell-mediated responses to a bystander antigen (ovalbumin [OVA]). MAIP-1 recognized only one of the three polypeptide chains of PAS-1, but neutralized the suppressive effect of the whole worm extract on OVA-specific antibody production. PAS-1 inhibited antibody production against a T-cell-dependent, but not a T-cell-independent, antigen in a dose-dependent way. IgM, IgG1, IgG2b, and also IgE and anaphylactic IgG1 levels were downregulated. In addition, PAS-1 inhibited OVA-specific delayed type hypersensitivity reactions in the footpad of mice, showing a potent immunosuppressive activity on both Th1 and Th2 responses that seems to be mediated by the induction of large amounts of IL-10 and IL-4. Indeed, PAS-1-specific spleen cells secreted sevenfold more IL-10 and threefold more IL-4 than OVA-specific cells in response to in vitro restimulation with the respective antigens. In conclusion, we showed that PAS-1, a single protein component from A. suum, maintains all its immunosuppressive properties. |
| Databáze: | OpenAIRE |
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