High-performance calcium sensors for imaging activity in neuronal populations and microcompartments
| Autor: | Boaz Mohar, Karel Svoboda, Loren L. Looger, Hod Dana, Ronak Patel, Brad K. Hulse, Douglas S. Kim, Vivek Jayaraman, Eric R. Schreiter, Allan M. Wong, Aaron M. Kerlin, John J. Macklin, Arthur Konnerth, Yang Chen, Yi Sun, Jeremy P. Hasseman, Getahun Tsegaye, Arthur Tsang |
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| Rok vydání: | 2018 |
| Předmět: |
Neurite
Green Fluorescent Proteins Neuromuscular Junction chemistry.chemical_element Calcium Biochemistry Green fluorescent protein 03 medical and health sciences Mice Calcium imaging Bacterial microcompartment medicine Neuropil Animals Molecular Biology Cells Cultured 030304 developmental biology Visual Cortex Neurons 0303 health sciences Cell Biology Rats medicine.anatomical_structure chemistry Biophysics Drosophila Female Neuron Preclinical imaging Biotechnology |
| Zdroj: | Nature methods. 16(7) |
| ISSN: | 1548-7105 |
| Popis: | Calcium imaging with genetically encoded calcium indicators (GECIs) is routinely used to measure neural activity in intact nervous systems. GECIs are frequently used in one of two different modes: to track activity in large populations of neuronal cell bodies, or to follow dynamics in subcellular compartments such as axons, dendrites and individual synaptic compartments. Despite major advances, calcium imaging is still limited by the biophysical properties of existing GECIs, including affinity, signal-to-noise ratio, rise and decay kinetics and dynamic range. Using structure-guided mutagenesis and neuron-based screening, we optimized the green fluorescent protein-based GECI GCaMP6 for different modes of in vivo imaging. The resulting jGCaMP7 sensors provide improved detection of individual spikes (jGCaMP7s,f), imaging in neurites and neuropil (jGCaMP7b), and may allow tracking larger populations of neurons using two-photon (jGCaMP7s,f) or wide-field (jGCaMP7c) imaging. |
| Databáze: | OpenAIRE |
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