Uptake of fluorescent plasmalogen analogs by cultured human skin fibroblasts deficient in plasmalogen
| Autor: | Josef Loidl, Fritz Paltauf, Eduard Paschke, Albin Hermetter, Georg Schwabe |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Plasmalogen
Plasmalogens Biophysics Phospholipid Fluorescence spectrometry Biochemistry Cerebrohepatorenal syndrome Fluorescence chemistry.chemical_compound medicine Choline Humans Cells Cultured Phospholipids Zellweger syndrome Vesicle Cell Membrane Biological Transport Cell Biology Peroxisome Fibroblasts medicine.disease chemistry lipids (amino acids peptides and proteins) Carrier Proteins |
| Zdroj: | Biochimica et biophysica acta. 1029(1) |
| ISSN: | 0006-3002 |
| Popis: | One of the consequences of hereditary peroxisomal dysfunction in the cerebro-hepato-renal (Zellweger) syndrome (CHRS) is a dramatic decrease in the biosynthesis and cellular content of ether lipids. In the present study effects of reduced cellular plasmalogen levels on membrane-membrane interactions were investigated. Cultured CHRS fibroblasts were incubated with unilamellar phospholipid vesicles consisting of 1-O-alkenyl-2-acyl- or 1,2-diacyl-sn-glycerophosphocholines and ethanolamines, carrying either the trans-parinaroyl or the 1,6-diphenyl-1,3,5-hexatriene propionyl group in position 2. Transfer of the fluorogenic phospholipids from vesicles to cells was followed by measuring the concomitant increase in fluorescence intensity. Transfer of phospholipids from cells to vesicles was monitored by incubating cells, prelabeled with [3H]oleic acid, in the presence of phospholipid vesicles. Fibroblasts from healthy donors or CHRS fibroblasts supplemented with the plasmalogen precursor 1-O-hexadecylglycerol served as controls. Plasmalogen-deficient cells exhibited a significantly increased tendency to take up exogenous choline or ethanolamine plasmalogens. Cellular plasmalogens were transferred from control cells to vesicles at a higher rate if the acceptor vesicles consisted of plasmalogens as compared to diacylglycerophosphocholine. Thus, it appears as if mechanisms existed which preserve cellular plasmalogen levels during interaction with exogenous phospholipid pools. Preliminary experimental evidence suggests that the observed exchange of phospholipids between cultured fibroblasts and vesicles occurs by a protein-catalyzed process. |
| Databáze: | OpenAIRE |
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